SAE1 Antibody
| Code | Size | Price |
|---|
| PSI-5749-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5749-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SAE1 Antibody: AOS1, SUA1, UBLE1A, HSPC140, AOS1, SUMO-activating enzyme subunit 1, Ubiquitin-like 1-activating enzyme E1A
Application Note:
SAE1 antibody can be used for detection of SAE1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
SAE1 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (SUMO-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. This sumoylation is effected by the heterodimeric enzyme SAE1/SAE2 and the SUMO-1-conjugating enzyme Ubch9. The sumoylation pathway mediated by SAE1/SAE2 is distinct from other ubiquitin-like protein (Ubl) pathways.
Background References:
- Kamitani T, Kito K, Nguyen HP, et al. Characterization of a second member of the sentrin family of ubiquitin-like proteins. J. Biol. Chem.1998;273:11349-53.
- Kim KI, Baek SH, and Chung CH. Versatile protein tag, SUMO: its enzymology and biological function. J. Cell. Physiol.2002; 191: 257-68.
- Desterro JM, Rodriguez MS, Kemp GD, et al. Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J. Biol. Chem.1999; 274:10618-24.
- Tatham MH, Jaffray E, Vaughan OA, et al. Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9. J. Biol. Chem.2001; 276:35368-74.
Buffer:
SAE1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (90%)
Immunogen:
SAE1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human SAE1.
The immunogen is located within amino acids 240 - 290 of SAE1.
The immunogen is located within amino acids 240 - 290 of SAE1.
NCBI Gene ID #:
10055
NCBI Official Name:
SUMO1 activating enzyme subunit 1
NCBI Official Symbol:
SAE1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 38 kDa
Observed: 39 kDa
Observed: 39 kDa
Protein Accession #:
NP_005491
Protein GI Number:
4885585
Purification:
SAE1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q9UBE0
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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