SAE2 Antibody

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ProSci
Product Code: PSI-5751
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5751-0.02mg0.02mg£150.00
Quantity:
PSI-5751-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of SAE2 in 293 cell lysate with SAE2 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunocytochemistry of SAE2 in 293 cells with SAE2 antibody at 4 μg/mL.
3 / 3
Immunofluorescence of SAE2 in 293 cells with SAE2 antibody at 20 μg/mL.
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Western blot analysis of SAE2 in 293 cell lysate with SAE2 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of SAE2 in 293 cells with SAE2 antibody at 4 μg/mL.
Immunofluorescence of SAE2 in 293 cells with SAE2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
SAE2 Antibody: ARX, SAE2, HRIHFB2115, UBLE1B, SUMO-activating enzyme subunit 2, Anthracycline-associated resistance ARX
Application Note:
SAE2 antibody can be used for detection of SAE2 by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SAE2 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (SUMO-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. This sumoylation is effected by the heterodimeric enzyme SAE1/SAE2 and the SUMO-1-conjugating enzyme Ubch9. The sumoylation pathway mediated by SAE1/SAE2 is distinct from other ubiquitin-like protein (Ubl) pathways.
Background References:
  • Kamitani T, Kito K, Nguyen HP, et al. Characterization of a second member of the sentrin family of ubiquitin-like proteins. J. Biol. Chem.1998;273:11349-53.
  • Kim KI, Baek SH, and Chung CH. Versatile protein tag, SUMO: its enzymology and biological function. J. Cell. Physiol.2002; 191: 257-68.
  • Desterro JM, Rodriguez MS, Kemp GD, et al. Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J. Biol. Chem.1999; 274:10618-24.
  • Tatham MH, Jaffray E, Vaughan OA, et al. Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9. J. Biol. Chem.2001; 276:35368-74.
Buffer:
SAE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (94%)
Immunogen:
SAE2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SAE2.

The immunogen is located within amino acids 550 - 600 of SAE2.
NCBI Gene ID #:
10054
NCBI Official Name:
ubiquitin-like modifier activating enzyme 2
NCBI Official Symbol:
UBA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005490
Protein GI Number:
4885649
Purification:
SAE2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q9UBT2
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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