SUMO2/3 Antibody
| Code | Size | Price |
|---|
| PSI-5755-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5755-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SUMO2/3 Antibody: HSMT3, SMT3B, SUMO3, Smt3A, SMT3H2, SMT3A, Small ubiquitin-related modifier 2, HSMT3, SUMO-2
Application Note:
SUMO2/3 antibody can be used for detection of SUMO2 and SUMO3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
SUMO2/3 Antibody: Small ubiquitin-like modifiers (SUMOs) are a family of small, related proteins (Sumo-1/2/3/4) that can be enzymatically attached to a target protein by a post-translational modification process termed sumoylation, which is a major regulator of protein function in cellular processes such as nuclear transport, transcriptional regulation, apoptosis and protein stability. All SUMO proteins localize to the nucleus and are covalently conjugated, affecting protein structure, function and interactions. SUMO2 and 3 are 96% identical and are more mobile within nucleus relative to SUMO1. Specific functional differences between SUMO1 and SUMO2 and 3 remain to be identified.
Background References:
- Kamitani T, Kito K, Nguyen HP, et al. Characterization of a second member of the sentrin family of ubiquitin-like proteins. J. Biol. Chem.1998;273:11349-53.
- Kim KI, Baek SH, and Chung CH. Versatile protein tag, SUMO: its enzymology and biological function. J. Cell. Physiol.2002; 191: 257-68.
- Su H and Li SS. Molecular features of human ubiquitin-like SUMO genes and their encoded proteins. Gene2002; 296: 65.
- Saitoh H and Hinchey J. Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3. J. Biol. Chem.2000; 275:6252-8.
Buffer:
SUMO2/3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (93%), Pig: (93%)
Immunogen:
SUMO2/3 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SUMO2.
The immunogen is located within the last 50 amino acids of SUMO2/3.
The immunogen is located within the last 50 amino acids of SUMO2/3.
NCBI Gene ID #:
6613
NCBI Official Name:
SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
NCBI Official Symbol:
SUMO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 15 kDa
Observed: 15 kDa
Observed: 15 kDa
Protein Accession #:
AAH71645
Protein GI Number:
47940079
Purification:
SUMO2/3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
P61956
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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