RNF8 Antibody

Product Code: PSI-5763

Supplier: ProSci

Code	Size	Price

PSI-5763-0.02mg

0.02mg

£150.00

Quantity:

PSI-5763-0.1mg

0.1mg

£449.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Applications:

Enzyme-Linked Immunosorbent Assay (ELISA)
Immunofluorescence (IF)
Western Blot (WB)

Images

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Western blot analysis of RNF8 in human lung tissue lysate with RNF8 antibody at (A) 1 and (B) 2 μg/mL.

2 / 3

Immunofluorescence of RNF8 in rat lung tissue with RNF8 antibody at 20 μg/mL.

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Documents

Further Information

Additional Names:

RNF8 Antibody: hRNF8, KIAA0646, E3 ubiquitin-protein ligase RNF8, RING finger protein 8, hRNF8

Application Note:

RNF8 antibody can be used for detection of RNF8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Background:

RNF8 Antibody: RNF8 was identified as a ubiquitin ligase (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A histone ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.

Background References:

Ito K, Adachi S, Iwakami R, et al. N-Terminally extended human ubiquitin-conjugating enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8. Eur. J. Biochem. 2001; 268:2725-32.
Mailand N, Bekker-Jensen S, Faustrup H, et al. RNF8 ubiquitylates histones at DNA double-strand breaks and promotes assembly of repair proteins. Cell 2007; 131:887-900.
Doil C, Mailand N, Bekker-Jensen S, et al. RNF168 binds and amplifies ubiquitin conjugates on damaged chromosomes to allow accumulation of repair proteins. Cell 2009; 136:435-46.
Ramachandran S, Chahwen R, Nepal RN, et al. The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination. Proc. Natl. Acad. Sci. USA 2010; 107:809-14.

Buffer:

RNF8 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration:

1 mg/mL

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Homology:

Predicted species reactivity based on immunogen sequence: Bovine: (92%)

Immunogen:

RNF8 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human RNF8.

The immunogen is located within amino acids 330 - 380 of RNF8.

NCBI Gene ID #:

9025

NCBI Official Name:

ring finger protein 8

NCBI Official Symbol:

RNF8

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

Predicted: 46, 53 kDa

Observed: 50 kDa

Protein Accession #:

NP_003949

Protein GI Number:

4504867

Purification:

RNF8 Antibody is affinity chromatography purified via peptide column.

Research Area:

Homeostasis

SPECIFICITY:

At least three isoforms of RNF8 are known to exist; this antibody will detect all three isoforms.

Swissprot #:

O76064

User NOte:

Optimal dilutions for each application to be determined by the researcher.

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