SIRT1 Antibody
| Code | Size | Price |
|---|
| PSI-5765-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5765-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Chicken
Antibody Isotype: IgY
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SIRT1 Antibody: SIR2L1, SIR2L1, NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, hSIRT1
Application Note:
SIRT1 antibody can be used for detection of SIRT1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Background:
SIRT1 Antibody: The Silent Information Regulator (SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and have important functions in the regulation of metabolism, growth and differentiation, inflammation, cellular survival, as well as in senescence and lifespan extension. Sirtuins, including SIRT1-7, are human homologs of yeast Sir2p. Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) which regulate cellular metabolism, e.g. energy metabolism, and thereby are associated with aging and several age-related diseases. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically via lysine residue.
Background References:
- Salminen A. SIRT1: regulation of longevity via autophagy.
- Afshar G and Murnane JP. Characterization of a human gene with sequence homology to Saccharomyces cerevisiae Sir 2. Gene1999; 234:161-8.
- Guarente L. Sirtuins as potential targets for metabolic syndrome. Nature2006; 444:868-74.
- Vaziri H, Dessain SK, Ng Eaton E, et al. hSIR2 (SIRT1) functions as an NAD-dependent p53 deacetylase. Cell2001; 107:149-59.
Buffer:
SIRT1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SIRT1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human SIRT1.
The immunogen is located within the first 50 amino acids of SIRT1.
The immunogen is located within the first 50 amino acids of SIRT1.
NCBI Gene ID #:
23411
NCBI Official Name:
sirtuin 1
NCBI Official Symbol:
SIRT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_036370
Protein GI Number:
7657575
Purification:
SIRT1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
Q96EB6
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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