B-raf Antibody

Contact us
ProSci
Product Code: PSI-5777
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5777-0.02mg0.02mg£150.00
Quantity:
PSI-5777-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Chicken
Antibody Isotype: IgY
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

1 / 3
<strong>Figure 1 Western Blot Validation in Human Brain Tissue</strong><br> Loading: 15 μg of lysates per lane. Antibodies: B-raf 5777, (A:1 μg/mL; B:2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution.
2 / 3
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: B-raf 5777, (0.5 μg/mL), B-raf 7379, (0.5 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-chicken (for 5777) and goat anti-rabbit (for 7379) IgG HRP conjugate at 1:10000 dilution.
3 / 3
<strong>Figure 3 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: B-raf 5777, (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
❮❯

<strong>Figure 1 Western Blot Validation in Human Brain Tissue</strong><br> Loading: 15 μg of lysates per lane. Antibodies: B-raf 5777, (A:1 μg/mL; B:2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: B-raf 5777, (0.5 μg/mL), B-raf 7379, (0.5 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-chicken (for 5777) and goat anti-rabbit (for 7379) IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: B-raf 5777, (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Documents

Further Information

Additional Names:
B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf
Application Note:
WB: 0.5-2 μg/mL.

Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
Background:
B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.
Background References:
  • McCubrey et al. Biophys. Acta 2007; 1773:1263-84.
  • Madhunapantula and Robertsonet al. Cancer Res.2008; 68:5-8.
  • Sarkozy et al. Hum. Mutat.2009; 30:695-702.
Buffer:
B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (93%)
Immunogen:
Anti-B-raf antibody (5777) was raised against a peptide corresponding to 18 amino acids near the center of human B-raf.

The immunogen is located within amino acids 330 - 380 of B-raf.
ISOFORMS:
Human B-raf has one isoform (766aa, 84kD). Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD). Rat B-raf has one isoform (712aa, 80kD). 5777 can detect in human B-raf and mouse isoform 1.
NCBI Gene ID #:
673
NCBI Official Name:
v-raf murine sarcoma viral oncogene homolog B1
NCBI Official Symbol:
BRAF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 94kD

Observed: 94 kD
Protein Accession #:
P15056
Protein GI Number:
50403720
Purification:
B-raf Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
P15056
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar B-raf expression profile in human and mouse cell lines detected by two independent anti-B-raf antibodies that recognize different epitopes, 5777 against central domain and 7379 against a slight different central domain.  B-raf proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Related Products

Product NameProduct CodeSupplier 
B-raf PeptidePSI-5777PProSci Summary Details