C-raf Antibody

Contact us
ProSci
Product Code: PSI-5779
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5779-0.02mg0.02mg£150.00
Quantity:
PSI-5779-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Chicken
Antibody Isotype: IgY
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

1 / 1
Western blot analysis of C-raf in A431 cell lysate with NPC1 antibody at (A) 0.5 and (B) 1 μg/mL.
❮❯

Western blot analysis of C-raf in A431 cell lysate with NPC1 antibody at (A) 0.5 and (B) 1 μg/mL.

Documents

Further Information

Additional Names:
C-raf Antibody: NS5, CRAF, Raf-1, c-Raf, RAF, Proto-oncogene c-RAF, cRaf
Application Note:
C-raf antibody can be used for detection of C-raf by Western blot at 0.5 - 1 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
C-raf Antibody: C-raf is the cellular homolog of viral raf gene (v-raf) and encodes a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases. Once activated, C-raf phosphorylates and activates the protein kinases MEK1 and MEK2, which in turn phosphorylate and activate the serine/threonine specific protein kinases, ERK1 and ERK2. These activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Background References:
  • Howe LR, Leevers SJ, Gomez N, et al. Activation of the MAP kinase pathway by the protein kinase raf. Cell1992; 71:335-42.
  • Chang F, Steelman LS, Shelton JG, et al. Regulation of cell cycle progression and apoptosis by the Ras/Raf/MEK/ERK pathway. Int. J. Oncol.2003; 22:469-80.
  • Pandit B, Sarkozy A, Pennacchio LA, et al. Gain-of-function RAF1 mutations cause Noonan and LEOPARD syndromes with hypertrophic cardiomyopathy. Nat. Genet.2007; 39:1007-12.
Buffer:
C-raf Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (84%)
Immunogen:
C-raf antibody was raised against an 19 amino acid synthetic peptide near the amino terminus of human C-raf.

The immunogen is located within amino acids 30 - 80 of C-raf.
NCBI Gene ID #:
5894
NCBI Official Name:
v-raf-1 murine leukemia viral oncogene homolog 1
NCBI Official Symbol:
RAF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 72 kDa

Observed: 72 kDa
Protein Accession #:
NP_002871
Protein GI Number:
4506401
Purification:
C-raf Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
P04049
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
C-raf PeptidePSI-5779PProSci Summary Details