FOXO1 Antibody
| Code | Size | Price |
|---|
| PSI-5811-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5811-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
FOXO1 Antibody: FKH1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A
Application Note:
FOXO1 antibody can be used for detection of FOXO1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
FOXO1 Antibody: FOXO1, also known as FKHR or ForkHead in Rhabdomyosarcoma, is a 70 kDa protein which is a ubiquitously expressed member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. In insulin-responsive tissues, stress or nutrient abundance triggers phosphorylation by PKB/AKT, blocking nuclear translocation and activity. Genetic mutations involving FOXO1A are a cause of alveolar rhabdomyosarcoma (RMS2). Recent studies link the anti-tumor activity of FOXO1 and the process of autophagy.
Background References:
- Anderson MJ, Viars CS, Czekay S, et al. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics1998; 47:187-99.
- Greer EL and Brunet A. FOXO transcription factors at the interface between longevity and tumor suppression. Oncogene2005; 24:7410-25.
- Brunet A, Bonni A, Zigmond MJ, et al. Akt promotes cell survival by phosphorylating and inhibiting a forkhead transcription factor. Cell1999; 96:857-68.
- Linardic CM. PAX3-FOXO1 fusion gene in rhabdomyosarcoma. Cancer Lett.2008; 270:10-8.
Buffer:
FOXO1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%)
Immunogen:
FOXO1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human FOXO1.
The immunogen is located within the first 50 amino acids of FOXO1.
The immunogen is located within the first 50 amino acids of FOXO1.
NCBI Gene ID #:
2308
NCBI Official Name:
forkhead box O1
NCBI Official Symbol:
FOXO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002006
Protein GI Number:
9257222
Purification:
FOXO1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
Swissprot #:
Q12778
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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