FOXO3 Antibody
| Code | Size | Price |
|---|
| PSI-5813-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5813-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
FOXO3 Antibody: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, Forkhead box protein O3, AF6q21 protein
Application Note:
FOXO3 antibody can be used for detection of FOXO3 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Background:
FOXO3 Antibody: FOXO3 is a ubiquitously expressed 75 kDa protein member of a subfamily of the forkhead homeotic gene family of transcription factors and shuttles between the cytoplasm and nucleus. FOXO transcription factors are key players of cell fate decisions, metabolism, stress resistance, tumor suppression and are regulated by growth factors, oxidative stress or nutrient deprivation. FOXO3 is involved with mTOR in the regulation of autophagy in skeletal muscle, and activates protein degradation in atrophying muscle cells. FOXO3 has also been implicated in several neurodegenerative disorders including aging, neuromuscular disease, systemic lupus erythmatosus, stroke and diabetic complications.
Background References:
- Anderson MJ, Viars CS, Czekay S, et al. Cloning and characterization of three human forkhead genes that comprise an FKHR-like gene subfamily. Genomics1998; 47:187-99.
- Greer EL and Brunet A. FOXO transcription factors at the interface between longevity and tumor suppression. Oncogene2005; 24:7410-25.
- Mammucari C, Schiaffino S, and Sandri M. Downstream of Akt: FoxO3 and mTOR in the regulation of autophagy in skeletal muscle. Autophagy2008; 4:524-6.
- Zhao J, Brault JJ, Schild A, et al. FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells. Cell Metab.2007; 6:472-83.
Buffer:
FOXO3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FOXO3 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human FOXO3.
The immunogen is located within amino acids 40 - 90 of FOXO3.
The immunogen is located within amino acids 40 - 90 of FOXO3.
NCBI Gene ID #:
2309
NCBI Official Name:
forkhead box O3
NCBI Official Symbol:
FOXO3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_963853
Protein GI Number:
42519916
Purification:
FOXO3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
Swissprot #:
O43524
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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