SIP1 Antibody

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ProSci
Product Code: PSI-5823
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5823-0.02mg0.02mg£150.00
Quantity:
PSI-5823-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of SIP1 in HeLa cell lysate with SIP1 antibody at (A) 0.5 and (B) 1μg/mL.
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Immunocytochemistry of SIP1 in HeLa cells with SIP1 antibody at 4 μg/mL.
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Immunofluorescence of SIP1 in HeLa cells with SIP1 antibody at 20 μg/mL.
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Western blot analysis of SIP1 in HeLa cell lysate with SIP1 antibody at (A) 0.5 and (B) 1μg/mL.
Immunocytochemistry of SIP1 in HeLa cells with SIP1 antibody at 4 μg/mL.
Immunofluorescence of SIP1 in HeLa cells with SIP1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
SIP1 Antibody: SIP1, SIP1-delta, SIP1, Gem-associated protein 2, Component of gems 2, Gemin-2
Application Note:
SIP1 antibody can be used for detection of SIP1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SIP1 Antibody: SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.
Background References:
  • Liu Q, Fischer U, Wang F, et al. The spinal muscle atrophy disease gene product, SMN, and its associated protein SIP1 are in a complex with spliceosomal snRNP proteins. Cell1997; 90:1013-21.
  • Ogawa C, Usui K, Aoki M, et al. Gemin2 plays an important role in stabilizing the survival of motor neuron complex. J. Biol. Chem.2007; 282:11122-34.
  • Jablonka S, Holtmann B, Meister G, et al. Gene targeting of Gemin2 in mice reveals a correlation between defects in the biogenesis of U snRNPs and motoneuron cell death. Proc. Natl. Acad. Sci. USA2002; 99:10126-31.
  • McWhorter ML, Boon KL, Horan ES, et al. The SMN binding protein Gemin2 is not involved in motor axon outgrowth. Dev. Neurobiol.2008; 68:182-94.
Buffer:
SIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SIP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human SIP1.

The immunogen is located within the last 50 amino acids of SIP1.
NCBI Gene ID #:
8487
NCBI Official Name:
gem nuclear organelle associated protein 2
NCBI Official Symbol:
GEMIN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAB82297
Protein GI Number:
2570925
Purification:
SIP1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
O14893
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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