ZEB1 Antibody
| Code | Size | Price |
|---|
| PSI-5825-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5825-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ZEB1 Antibody: BZP, TCF8, AREB6, FECD6, NIL2A, PPCD3, ZFHEP, ZFHX1A, DELTAEF1, Zinc finger E-box-binding homeobox 1, NIL-2-A zinc finger protein, TCF-8
Application Note:
ZEB1 antibody can be used for detection of ZEB1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ZEB1 Antibody: ZEB1, initially identified as the delta-crystallin enhancer binding protein delta EF1, is a DNA-binding protein that binds to a modified E-box sequence and has been implicated in postgastrulation embryogenesis. ZEB1 binds to the promoter of several hemapoietic genes, including interleukin-2, CD4, GATA-3, and alpha-integrin, and mice in which ZEB1 has been targeted show thymic atrophy, and severe defects in lymphocyte differentiation. Recent evidence suggests that ZEB1 also regulates the accumulation of adipose tissue and may play a role in obesity. Mutations in this gene have been associated with late-onset Fuchs endothelial corneal dystrophy.
Background References:
- Funahashi J, Sekido R, Murai K, et al. Delta-crystallin enhancer protein delta EF1 is a zinc finger-homeodomain protein implicated in postgastrulation embryogenesis. Dev.1993; 119:433-46.
- Postigo AA and Dean DC. Independent repressor domains in ZEB regulate muscle and T-cell differentiation. Mol. Cell Biol.1999; 19:7961-71.
- Saykally JN, Dogan S, Cleary MP, et al. The ZEB1 transcription factor is a novel repressor of adiposity in female mice. PLos One2009; 4:e8460.
- Mehta JS, Vithana EN, Tan DT, et al. Analysis of the posterior polymorphous corneal dystrophy 3 gene, TCF8, in late-onset Fuchs endothelial corneal dystrophy. Invest. Opthalmol. Vis. Sci.2008; 49:184-8.
Buffer:
ZEB1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ZEB1 antibody was raised against a 15 amino acid synthetic peptide near the center of human ZEB1.
The immunogen is located within amino acids 530 - 580 of ZEB1.
The immunogen is located within amino acids 530 - 580 of ZEB1.
NCBI Gene ID #:
6935
NCBI Official Name:
zinc finger E-box binding homeobox 1
NCBI Official Symbol:
ZEB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 124 kDa
Observed: 130 kDa
Observed: 130 kDa
Protein Accession #:
NP_110378
Protein GI Number:
55957123
Purification:
ZEB1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
Multiple isoforms of ZEB1 are known to exist.
Swissprot #:
P37275
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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