FREM1 Antibody

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ProSci
Product Code: PSI-5829
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5829-0.02mg0.02mg£150.00
Quantity:
PSI-5829-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1μg/mL.
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Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.
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Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.
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Western blot analysis of FREM1 in K562 cell lysate with FREM1 antibody at (A) 0.5 and (B) 1μg/mL.
Immunocytochemistry of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.
Immunofluorescence of FREM1 in K562 cells with FREM1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
FREM1 Antibody: BNAR, MOTA, TILRR, TRIGNO2, C9orf143, C9orf145, C9orf154, FRAS1-related extracellular matrix protein 1, Protein QBRICK
Application Note:
FREM1 antibody can be used for detection of FREM1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
FREM1 Antibody: FREM1 is a member of the FRAS1-related extracellular matrix protein family and is thought to play a role in craniofacial and renal development. FREM1 functions as an extracellular matrix protein that is essential for epidermal adhesion during embryogenesis and may also participate in epidermal differentiation. It is recognized by cells in the embryonic skin and hair follicles through different members of the integrin family. Deficiency in the Fras1/Frem genes gives rise to the bleb phenotype, which is equivalent to the human hereditary disorder Fraser syndrome.
Background References:
  • Smyth I, Du X, Taylor MS, et al. The extracellular matrix gene FREM1 is essential for the normal adhesion of the embryonic epidermis. Proc. Natl. Acad. Sci. USA2004; 101:13560-5.
  • Kiyozumi D, Osada A, Sugimoto N, et al. Identification of a novel cell-adhesive protein spatiotemporally expressed in the basement membrane of mouse developing hair follicle. Exp. Cell Res.2005; 306:9-23.
  • Petrou P, Makrygiannis AK, Chalepakis G, et al. The FRAS1/FREM family of extracellular matrix proteins: structure, function, and association with Fraser syndrome and the mouse bleb phenotype. Connect. Tissue Res.2008; 49:277-82.
  • Zhang X, Shephard F, Kim HB, et al. TILRR, a novel IL-1RI co-receptor, potentiates MyD88 recruitment to control Ras-dependent amplification of NF-kappaB J. Biol. Chem.2010; 285:7222-32.
Buffer:
FREM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FREM1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human FREM1.

The immunogen is located within the last 50 amino acids of FREM1.
NCBI Gene ID #:
158326
NCBI Official Name:
FRAS1 related extracellular matrix 1
NCBI Official Symbol:
FREM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_659403
Protein GI Number:
122056683
Purification:
FREM1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
Q5H8C1
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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