FREM2 Antibody

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ProSci
Product Code: PSI-5831
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5831-0.02mg0.02mg£150.00
Quantity:
PSI-5831-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of FREM2 in A-20 cell lysate with FREM2 antibody at (A) 0.5 and (B) 1μg/mL.
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Immunocytochemistry of FREM2 in A-20 cells with FREM2 antibody at 20 μg/mL.
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Immunofluorescence of FREM2 in A20 cells with FREM2 antibody at 20 μg/mL.
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Western blot analysis of FREM2 in A-20 cell lysate with FREM2 antibody at (A) 0.5 and (B) 1μg/mL.
Immunocytochemistry of FREM2 in A-20 cells with FREM2 antibody at 20 μg/mL.
Immunofluorescence of FREM2 in A20 cells with FREM2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
FREM2 Antibody: FRAS1-related extracellular matrix protein 2, ECM3 homolog
Application Note:
FREM2 antibody was raised against a 19 amino acid peptide near the center of human FREM2. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
FREM2 Antibody: FREM2 is a member of the FRAS1-related extracellular matrix protein family and like FREM1, mutations in the FREM2 gene are associated with Fraser syndrome. FREM2 localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions during morphogenetic processes and is thought to be required for maintaining the integrity of the skin epithelium and the differentiated state of renal epithelia. The FREM2 gene is one of several genes whose transcription is affecteded by TFAP2C, a transcription factor involved in mammary development, differentiation, and oncogenesis.
Background References:
  • Jadjeda S, Smyth I, Pitera JE, et al. Identification of a new gene mutated in Fraser syndrome and mouse myelencephalic blebs. Nat. Genet.2005; 37:520-5.
  • Pavlakis E, Makrygiannis AK, Chiokati R, et al. Differential localization profile of Fras1/Frem proteins in epithelial basement membranes of newbord and adult mice. Histochem. Cell Biol.2008; 130:785-93.
  • Timmer JR, Mak TW, Manova K, et al. Tissue morphogenesis and vascular stability require the Frem2 protein, product of the mouse myelencephalic blebs gene. Proc. Natl. Acad. Sci. USA2005; 102:11746-50.
  • Woodfield GW, Chen Y, Bair TB, et al. Identification of primary gene targets of TFAP2C in hormone responsive breast carcinoma cells. Genes Chromosomes Cancer2010; epub.
Buffer:
FREM2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FREM2 antibody was raised against a 19 amino acid synthetic peptide near the center of human FREM2.

The immunogen is located within amino acids 1920 - 1970 of FREM2.
NCBI Gene ID #:
341640
NCBI Official Name:
FRAS1 related extracellular matrix protein 2
NCBI Official Symbol:
FREM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_997244
Protein GI Number:
79749430
Purification:
FREM2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
Q5SZK8
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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