ZC3HAV1 Antibody

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ProSci
Product Code: PSI-5839
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5839-0.02mg0.02mg£150.00
Quantity:
PSI-5839-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of ZC3HAV1 in HeLa cell lysate with ZC3HAV1 antibody at 1μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of ZC3HAV1 in HeLa cells with ZC3HAV1 antibody at 20 μg/mL.
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Immunofluorescence of ZC3HAV1 in Hela cells with ZC3HAV1 antibody at 20 μg/mL.
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Western blot analysis of ZC3HAV1 in HeLa cell lysate with ZC3HAV1 antibody at 1μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of ZC3HAV1 in HeLa cells with ZC3HAV1 antibody at 20 μg/mL.
Immunofluorescence of ZC3HAV1 in Hela cells with ZC3HAV1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
ZC3HAV1 Antibody: ZAP, ZC3H2, ARTD13, PARP13, FLB6421, ZC3HDC2, PRO1677, Zinc finger CCCH-type antiviral protein 1, ADP-ribosyltransferase diphtheria toxin-like 13
Application Note:
ZC3HAV1 antibody can be used for detection of ZC3HAV1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ZC3HAV1 Antibody: The zinc finger antiviral protein (ZC3HAV1) is a CCCH type zinc finger protein that induces an innate immunity to infections by retrovirus by preventing the accumulation of viral RNAs in the cytoplasm and recruits the RNA processing exosome to degrade target RNAs, thereby inhibiting virus replication. ZC3HAV1 is localized in the cytoplasm at steady state, but shuttles between nucleus and cytoplasm in a XPO1-dependent manner. ZAP is a direct target gene of IRF3 action in cellular antiviral responses.
Background References:
  • Gao G, Guo X, and Goff SP. Inhibition of retroviral RNA production by ZAP, a CCCH-Type zinc finger protein. Science2002; 297:1703-6.
  • Guo X, Ma J, Sun J, et al. The zinc-finger antiviral protein recruits the RNA processing exosome to degrade the target mRNA. Proc. Natl. Acad. Sci. USA2007; 104:151-6.
  • MacDonald MR, Machlin ES, Albin OR, et al. The zinc finger antiviral protein acts synergistically with an interferon-induced factor for maximal activity against alphaviruses. J. Virol. 2007; 81:13509-18.
  • Wang N, Dong Q, Li J, et al. Viral induction of the zinc finger antiviral protein is IRF3-dependent but NF-kappaB-independent. J. Biol. Chem.2010; 285:6080-90.
Buffer:
ZC3HAV1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ZC3HAV1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human ZC3HAV1.

The immunogen is located within amino acids 680 - 730 of ZC3HAV1.
NCBI Gene ID #:
56829
NCBI Official Name:
zinc finger CCCH-type, antiviral 1
NCBI Official Symbol:
ZC3HAV1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_078901
Protein GI Number:
27477138
Purification:
ZC3HAV1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
Swissprot #:
Q7Z2W4
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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