USP10 Antibody

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ProSci
Product Code: PSI-5803
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5803-0.02mg0.02mg£150.00
Quantity:
PSI-5803-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of USP10 in Jurkat cell lysate with USP10 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunocytochemistry of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.
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Immunofluorescence of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.
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Western blot analysis of USP10 in Jurkat cell lysate with USP10 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.
Immunofluorescence of USP10 in Jurkat cells with USP10 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
USP10 Antibody: UBPO, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10, Deubiquitinating enzyme 10
Application Note:
USP10 antibody can be used for detection of USP10 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
USP10 Antibody: USP10, also known as ubiquitin specific peptidase 10, belongs to the ubiquitin-specific protease family of cysteine proteases. USP10 functions to catalyze the cleavage of ubiquitin from ubiquitin-conjugated protein substrates such as p53/TP53, SNX3 and CFTR. USP10 has been identified as a subunit of DNA-bound androgen receptor (AR) complexes and may play a role in the activity of the DNA-bound androgen receptor complex. USP10 also acts as an essential regulator of p53/TP53 stability and is thought to function as a tumor suppressor.
Background References:
  • D'Andrea A and Pellman D. Deubiquitinating enzymes: a new class of biological regulators. Crit. Rev. Biochem. Mol. Biol.1998; 33:337-52.
  • Bomberger JM, Barnaby RL, and Stanton BA. The deubiquitinating enzyme USP10 regulates the endocytic recycling of CFTR in airway epithelial cells. Channels2010; 4(3).
  • Faus H, Meyer HA, Huber M, et al. The ubiquitin-specific protease USP10 modulates androgen receptor function. Mol. Cell. Endocrinol.2005; 245:138-46.
  • Yuan J, Luo K, Zhang L, et al. USP10 regulates p53 localization and stability by deubiquitinating p53. Cell 2010; 140:384-96.
Buffer:
USP10 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (80%), Rat: (91%), Chicken: (89%)
Immunogen:
USP10 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human USP10.

The immunogen is located within amino acids 30 - 80 of USP10.
NCBI Gene ID #:
9100
NCBI Official Name:
ubiquitin specific peptidase 10
NCBI Official Symbol:
USP10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005144
Protein GI Number:
119220605
Purification:
USP10 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q14694
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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