SCUBE2 Antibody

Contact us
ProSci
Product Code: PSI-5845
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5845-0.02mg0.02mg£150.00
Quantity:
PSI-5845-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of SCUBE2 in Daudi cell lysate with SCUBE2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 3
Immunocytochemistry of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.
3 / 3
Immunofluorescence of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.
❮❯

Western blot analysis of SCUBE2 in Daudi cell lysate with SCUBE2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.
Immunofluorescence of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
SCUBE2 Antibody: CEGB1, CEGF1, CEGP1, Signal peptide, CUB and EGF-like domain-containing protein 2, Protein CEGP1
Application Note:
SCUBE2 antibody can be used for detection of SCUBE2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SCUBE2 Antibody: SCUBE2 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and are expressed in embryonic neuroectoderm and vascular epithelium. SCUBE2 is a close homolog to SCUBE1 and can form a heterodimeric complex with SCUBE1. SCUBE2 is thought to participate in the Hedgehog signaling pathway by specifically interacting with Sonic Hedgehog (SHH) and the receptor PTCH1, enhancing the SHH signaling activity in the raft microdomains of the plasma membrane, and may thus play important roles in organ development and tumor progression.
Background References:
  • Grimmond S, Larder R, Van Hateren N, et al. Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1). Genomics2000; 70:74-81.
  • Yang RB, Ng CK, Wasserman SM, et al. Identification of a novel family of cell-surface proteins expressed in human vascular endothelium. J. Biol. Chem.2002; 277:46364-73.
  • Grimmond S, Larder R, Van Hateren N, et al. Cloning, mapping, and expression analysis of a gene encoding a novel mammalian EGF-related protein (SCUBE1). Genomics2000; 70:74-81.
  • Tsai M, Cheng C, Lin Y, et al. Isolation and characterization of a secreted, cell-surface glycoprotein SCUBE2 from humans. Biochem. J. 2009; 422:119-28.
Buffer:
SCUBE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SCUBE2 antibody was raised against a 17 amino acid synthetic peptide near the center of human SCUBE2.

The immunogen is located within amino acids 480 - 530 of SCUBE2.
NCBI Gene ID #:
57758
NCBI Official Name:
signal peptide, CUB domain, EGF-like 2
NCBI Official Symbol:
SCUBE2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_066025
Protein GI Number:
283046663
Purification:
SCUBE2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
Q9NQ36
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
SCUBE2 PeptidePSI-5845PProSci Summary Details