ALKBH2 Antibody
| Code | Size | Price |
|---|
| PSI-5871-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-5871-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ALKBH2 Antibody: ABH2, ABH2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, Alkylated DNA repair protein alkB homolog 2
Application Note:
ALKBH2 antibody can be used for detection of ALKBH2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
ALKBH2 Antibody: The E. coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA; ALKBH2 and ALKBH3 are mammalian homologs of AlkB that catalyze the removal of 1-methyladenine and 3-methylcytosine, modifications that left unchecked could lead to cancerous cells. Mutations in both ALKBH2 and ALKBH3 have been observed in pediatric brain tumors indicating that these proteins are important in the prevention of cancer formation. Like the histone demethylase JMJD1A, ALKBH2 is a non-heme iron enzyme that is inhibited by Nickel ions, suggesting that inhibition of ALKBH2 by Nickel ions may play a role in the development of cancer. Conversely, ALKBH2 mRNA and protein levels are increased glioma cells following Photofrin-mediated photodynamic therapy, an adjuvant therapy in cancer treatment, suggesting that down-regulating ALKBH2 expression in cancer cells may enhance the anti-cancer effectiveness of this treatment.
Background References:
- Duncan T, Trewick SC, Koivisto P, et al. Reversal of DNA alkylation damage by two human dioxygenases. Proc. Natl. Acad. Sci. USA2002; 99:16660-5.
- Cetica V, Genitori L, Giunti L, et al. Pediatric brain tumors: mutations of two dioxygenases (hABH2 and hABH3) that directly repair alkylation damage. J. Neurooncol.2009; 195-201.
- Chen H, Giri NC, Zhang R, et al. Nickel ions inhibit histone demethylase JMJD1A and DNA repair enzyme ABH2 by replacing the ferrous iron in the catalytic centers. J. Biol. Chem.2010; 285:7374-83.
- Lee SY, Luk SK, Chuang CP, et al. TP53 regulates human AlkB homologue 2 expression in glioma resistance to Photofrin-mediated photodynamic therapy. Br. J. Cancer2010; 103:362-9.
Buffer:
ALKBH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
ALKBH2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ALKBH2.
The immunogen is located within amino acids 180 - 230 of ALKBH2.
The immunogen is located within amino acids 180 - 230 of ALKBH2.
NCBI Gene ID #:
121642
NCBI Official Name:
alkB, alkylation repair homolog 2 (E. coli)
NCBI Official Symbol:
ALKBH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001138847
Protein GI Number:
224451107
Purification:
ALKBH2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q6NS38
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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