TRIM71 Antibody

Contact us
ProSci
Product Code: PSI-5881
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5881-0.02mg0.02mg£150.00
Quantity:
PSI-5881-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of TRIM71 in human brain tissue lysate with TRIM71 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunohistochemistry of TRIM71 in human brain tissue with TRIM71 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of TRIM71 in Human Brain cells with TRIM71 antibody at 20 μg/mL.
❮❯

Western blot analysis of TRIM71 in human brain tissue lysate with TRIM71 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TRIM71 in human brain tissue with TRIM71 antibody at 5 μg/mL.
Immunofluorescence of TRIM71 in Human Brain cells with TRIM71 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
TRIM71 Antibody: LIN41, LIN-41, LIN41, E3 ubiquitin-protein ligase TRIM71, Protein lin-41 homolog
Application Note:
TRIM71 antibody can be used for detection of TRIM71 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TRIM71 Antibody: TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7.
Background References:
  • Lancman JJ, Caruccio NC, Harfe BD, et al. Analysis of the regulation of lin-41 during chick and mouse limb development. Dev. Dyn.2005; 234:948-60.
  • Lin YC, Hsieh LC, Kuo MW, et al. Human TRIM71 and its nematode homologue are targets of let-7 microRNA and its zebrafish orthologue is essential for development. Mol. Biol. Evol.2007; 24:2525-34.
  • Rybak A, Fuchs H, Hadian K, et al. The let-7 target gene mouse lin-41 is a stem cell specific E3 ubiquitin ligase for the miRNA pathway protein Ago2. Nat. Cell Biol.2009; 11:1411-20.
Buffer:
TRIM71 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Chicken: (100%)
Immunogen:
TRIM71 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human TRIM71.

The immunogen is located within amino acids 680 - 730 of TRIM71.
NCBI Gene ID #:
131405
NCBI Official Name:
tripartite motif-containing 71
NCBI Official Symbol:
TRIM71
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001034200
Protein GI Number:
84993742
Purification:
TRIM71 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
Q2Q1W2
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
TRIM71 PeptidePSI-5881PProSci Summary Details