KPNA4 Antibody

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ProSci
Product Code: PSI-5987
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5987-0.02mg0.02mg£150.00
Quantity:
PSI-5987-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of KPNA4 in HeLa cell lysate with KPNA4 antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 3
Immunocytochemistry of KPNA4 in HeLa cells with KPNA4 antibody at 2.5 μg/mL.
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Immunofluorescence of KPNA4 in HeLa cells with KPNA4 antibody at 20 μg/mL.
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Western blot analysis of KPNA4 in HeLa cell lysate with KPNA4 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of KPNA4 in HeLa cells with KPNA4 antibody at 2.5 μg/mL.
Immunofluorescence of KPNA4 in HeLa cells with KPNA4 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
KPNA4 Antibody: QIP1, SRP3, IPOA3, QIP1, Importin subunit alpha-3, Importin alpha Q1, Qip1
Application Note:
KPNA4 antibody can be used for detection of KPNA4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
KPNA4 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. Molecules containing the classical nuclear localization signal (NLS) are transported into the nucleus by alpha/beta heterodimers. KPNA3 has been shown to be important in the TNF-α-induced nuclear import of NF-κB. It is also involved in the stress-mediated nuclear stabilization of p53 and the nuclear import and replication of HIV-1 in both dividing and non-dividing cells.
Background References:
  • Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
  • Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
  • Goldfarb DS, Corbett AH, Mason DA, et al. Importin alpha: a multi-purpose nuclear-transport receptor. Trends Cell Biol.2004; 14:505-14.
  • Fagerlund R, Melen K, Cao X, et al. NF-kappaB p52, RelB, and c-Rel are transported into the nucleus via a subset of importin alpha molecules. Cell Signal.2008; 20:1442-51.
Buffer:
KPNA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
KPNA4 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA4.

The immunogen is located within amino acids 30 - 80 of KPNA4.
NCBI Gene ID #:
3840
NCBI Official Name:
karyopherin alpha 4 (importin alpha 3)
NCBI Official Symbol:
KPNA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
EAW78632
Protein GI Number:
119599038
Purification:
KPNA4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
O00629
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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