KPNA3 Antibody

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ProSci
Product Code: PSI-5985
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5985-0.02mg0.02mg£150.00
Quantity:
PSI-5985-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of KPNA3 in EL4 cell lysate with KPNA3 antibody at (A) 1 and (B) 2 μg/mL.
2 / 3
Immunocytochemistry of KPNA3 in HeLa cells with KPNA3 antibody at 10 μg/mL.
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Immunofluorescence of KPNA3 in EL4 cells with KPNA3 antibody at 20 μg/mL.
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Western blot analysis of KPNA3 in EL4 cell lysate with KPNA3 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of KPNA3 in HeLa cells with KPNA3 antibody at 10 μg/mL.
Immunofluorescence of KPNA3 in EL4 cells with KPNA3 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
KPNA3 Antibody: SRP1, SRP4, IPOA4, hSRP1, SRP1gamma, QIP2, Importin subunit alpha-4, Importin alpha Q2, Qip2
Application Note:
KPNA3 antibody can be used for detection of KPNA3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
KPNA3 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA3, the alpha subunit 3 of karyopherin, is silimar to human KPNA2, suggesting that KPNA3 may be involved in the nuclear transport system. It is ubiquitously expressed and recent studies have shown that human KPNA3 may be associated with schizophrenia.
Background References:
  • Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
  • Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
  • Takeda S, Fujiwara T, Shimizu F, et al. Isolation and mapping of karyopherin alpha 3 (KPNA3), a human gene that is highly homologous to genes encoding Xenopus importin, yeast SRP1 and human RCH1. Cytogenet. Cell Genet.1997; 76:87-93.
  • Zhang H, Ju G, Wei J, et al. A combined effect of the KPNA3 and KPNB3 genes on susceptibility to schizophrenia. Neurosci. Lett.2006; 402:173-5.
Buffer:
KPNA3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
KPNA3 antibody was raised against a 14 amino acid synthetic peptide near the carboxy end of human KPNA3.

The immunogen is located within the last 50 amino acids of KPNA3.
NCBI Gene ID #:
3839
NCBI Official Name:
karyopherin alpha 3 (importin alpha 4)
NCBI Official Symbol:
KPNA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002258
Protein GI Number:
34485722
Purification:
KPNA3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
O00505
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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