KPNA6 Antibody

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ProSci
Product Code: PSI-5991
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5991-0.02mg0.02mg£150.00
Quantity:
PSI-5991-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of KPNA6 in 293 cell lysate with KPNA6 antibody at 1 μg/mL.
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Immunofluorescence of KPNA6 in 293 cells with KPNA6 antibody at 20 μg/mL.
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Western blot analysis of KPNA6 in 293 cell lysate with KPNA6 antibody at 1 μg/mL.
Immunofluorescence of KPNA6 in 293 cells with KPNA6 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
KPNA6 Antibody: IPOA7, KPNA7, IPOA7, Importin subunit alpha-7, Karyopherin subunit alpha-6
Application Note:
KPNA6 antibody can be used for detection of KPNA6 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
KPNA6 Antibody: Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA6 belongs to a subfamily within the KPNA family that also includes IPOA5&6. Down-regulation of KPNA6 by RNAi expression in HeLa cells strongly inhibited cell proliferation, possibly due to blocking the nuclear import of specific factors essential for cell growth and proliferation. KPNA6 also interacts with the Rev protein of HIV-1 and promote its nuclear import.
Background References:
  • Moroianu J. Molecular mechanisms of nuclear protein transport. Crit. Rev. Eukaryot. Gene Expr.1997; 7:61-72.
  • Gilchrist D and Rexach M. Molecular basis for the rapid dissociation of nuclear localization signals from karyopherin alpha in the nucleoplasm. J. Biol. Chem.2003; 278: 51937-49.
  • Kohler MC, Fiebeler M, Hartwig S, et al. Evidence for distinct substrate specificity of importin alpha family members in nuclear protein import. Mol. Cell. Biol.1999; 19:7782-91.
  • Quensal C, Friedrich B, Sommer T, et al. In vivo analysis of importin a proteins reveals cellular proliferation inhibition and substrate specificity. Mol. Cell. Biol.2004; 24:10246-55.
Buffer:
KPNA6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (92%)
Immunogen:
KPNA6 antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of human KPNA6.

The immunogen is located within amino acids 70 - 120 of KPNA6.
NCBI Gene ID #:
23633
NCBI Official Name:
karyopherin alpha 6 (importin alpha 7)
NCBI Official Symbol:
KPNA6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
CAH71948
Protein GI Number:
55664992
Purification:
KPNA6 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
Swissprot #:
O60684
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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