TRIP6 Antibody

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ProSci
Product Code: PSI-6001
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6001-0.02mg0.02mg£150.00
Quantity:
PSI-6001-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TRIP6 in A431 cell lysate with TRIP6 antibody at 1 μg/mL.
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Immunocytochemistry of TRIP6 in HeLa cells with TRIP6 antibody at 10 μg/mL.
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Immunofluorescence of TRIP6 in HeLa cells with TRIP6 antibody at 20 μg/mL.
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Western blot analysis of TRIP6 in A431 cell lysate with TRIP6 antibody at 1 μg/mL.
Immunocytochemistry of TRIP6 in HeLa cells with TRIP6 antibody at 10 μg/mL.
Immunofluorescence of TRIP6 in HeLa cells with TRIP6 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
TRIP6 Antibody: OIP1, OIP-1, ZRP-1, TRIP-6, TRIP6i2, OIP1, Thyroid receptor-interacting protein 6, Opa-interacting protein 1, TR-interacting protein 6
Application Note:
TRIP6 antibody can be used for detection of TRIP6 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TRIP6 Antibody: TRIP6 (Thyroid receptor interacting protein 6) is a zyxin-related focal adhesion molecule involved in cell motility and antiapoptotic responses. TRIP6 is predominantly expressed in kidney, liver and lung. It has three LIM domains, a PDZ-binding motif, a Crk SH2-binding motif and/or other protein interacting domains. TRIP6 regulates NF-κB activation and interacts with RICK and TRAF2, proteins that are involved in TNF signaling. TRIP6 antagonizes Fas-induced apoptosis and promotes Fas-mediated cell migration in apoptosis-resistant glioma cells via Src-dependent phosphorylation of TRIP6 at Tyr-55.
Background References:
  • Murthy KK, Clark K, Fortin Y, et al. ZRP-1, a zyxin-related protein, interacts with the second PDZ domain of the cytosolic protein tyrosine phosphatase hPTP1E. J. Biol. Chem.1999; 274:20679-87.
  • Kassel O, Schneider S, Heilbock C, et al. A nuclear isoform of the focal adhesion LIM-domain protein Trip6 integrates activating and repressing signals at AP-1- and NF-kappaB regulated promoters. Genes Dev.2004; 18:2518-2528.
  • Lai YJ, Chen CS, Lin WJ, et al. c-Src-mediated phosphorylation of TRIP6 regulates its function in lysophosphatidic acid-induced cell migration. Mol. Cell. Biol.2005; 25:5859-5868.
  • Li L, Bin LH, Li F, et al. TRIP6 is a RIP2-associated common signaling component of multiple NF-kB activation pathways. J. Cell. Sci.2005; 118:555-563.
Buffer:
TRIP6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (86%)
Immunogen:
TRIP6 antibody was raised against a 15 amino acid synthetic peptide near the center of human TRIP6.

The immunogen is located within amino acids 190 - 240 of TRIP6.
NCBI Gene ID #:
7205
NCBI Official Name:
thyroid hormone receptor interactor 6
NCBI Official Symbol:
TRIP6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_003293
Protein GI Number:
91208423
Purification:
TRIP6 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
Swissprot #:
Q15654
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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