PLAC3 Antibody

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ProSci
Product Code: PSI-6019
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6019-0.02mg0.02mg£150.00
Quantity:
PSI-6019-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of PLAC3 in HeLa cell lysate with PLAC3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of PLAC3 in HeLa cells with PLAC3 antibody at 10 μg/mL.
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Immunofluorescence of PLAC3 in HeLa cells with PLAC3 antibody at 20 μg/mL.
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Western blot analysis of PLAC3 in HeLa cell lysate with PLAC3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of PLAC3 in HeLa cells with PLAC3 antibody at 10 μg/mL.
Immunofluorescence of PLAC3 in HeLa cells with PLAC3 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
PLAC3 Antibody: PAPPE, PLAC3, PAPP-E, PAPP-A2, Pappalysin-2, Pregnancy-associated plasma protein A2
Application Note:
PLAC3 antibody can be used for detection of PLAC3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PLAC3 Antibody: PLAC3 (placenta-specific protein 3), also known as PAPP-A2, is a metalloproteinase that cleaves the insulin-like growth factor-binding protein (IGFBP)-5 in a IGF-independent fashion. It has been suggested that the cleavage of IGFBP-5 and to a lesser extent IGFBP-3 produced in the maternal decidua promotes feto-placental growth. Recent studies have shown elevated levels of PLAC3 in hypertensive disorders of pregnancy including preeclampsia and HELLP (hemolysis, elevated liver enzymes, and low platelets) syndrome.
Background References:
  • Overgaard MT, Boldt HB, Laursen LS, et al. Pregnancy-associated plasma protein-A2 (PAPP-A2), a novel insulin-like growth factor-binding protein-5 proteinase. J. Biol. Chem.2001; 276:1849-53.
  • Wang J, Qiu Q, Haider M, et al. Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse. J. Endocrin.2009; 202:337-45.
  • Nishizawa H, Pryor-Koishi K, Suzuki M, et al. Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients. Mol. Hum. Reprod.2008; 14:595-602.
  • Buimer M, Keijser R, Jebbink JM, et al. Seven placental transcripts characterize HELLP syndrome. Placenta2008; 29:444-53.
Buffer:
PLAC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PLAC3 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human PLAC3.

The immunogen is located within amino acids 450 - 500 of PLAC3.
NCBI Gene ID #:
60676
NCBI Official Name:
pappalysin 2
NCBI Official Symbol:
PAPPA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_064714
Protein GI Number:
116174738
Purification:
PLAC3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q9BXP8
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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