PIWI-L1 Antibody
| Code | Size | Price |
|---|
| PSI-6029-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6029-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PIWI-L1 Antibody: HIWI, MIWI, PIWI, CT80.1, HIWI, Piwi-like protein 1
Application Note:
PIWI-L1 antibody can be used for detection of PIWI-L1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
PIWI-L1 Antibody: PIWI-L1 is a member of the PIWI subfamily of Argonaute proteins, evolutionarily conserved proteins containing both PAZ and Piwi motifs that are crucial for the biogenesis and function of small non-coding RNAs and play important roles in stem cell self-renewal, RNA silencing, and translational regulation in diverse organisms. PIWI-L1 is thought to play a role as an intrinsic regulator of the self-renewal capacity of germline and hematopoietic stem cells as removing PIWI-L1 protein from single germline stem cells significantly decreases the rate of their division. Recent studies have shown that overexpression of PIWI-L1 (as well as other PIWI-like proteins) are potential biomarkers for colon and other cancers.
Background References:
- Thomson T and Lin H. The biogenesis and function PIWI proteins and piRNAs: progress and prospect. Annu. Rev. Cell Dev. Biol.2009; 25:355-76.
- Cox DN, Chao A, and Lin H. PIWI encodes a nucleoplasmic factor whose activity modulates the number and division rate of germline stem cells. Development2000; 503-14.
- Li L, Yu C, Gao H, et al. Argonaute proteins: potential biomarkers for human colon cancer. BMC Cancer2010; 10:38.
Buffer:
PIWI-L1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (94%)
Immunogen:
PIWI-L1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PIWI-L1.
The immunogen is located within amino acids 60 - 110 of PIWI-L1.
The immunogen is located within amino acids 60 - 110 of PIWI-L1.
NCBI Gene ID #:
9271
NCBI Official Name:
piwi-like 1 (Drosophila)
NCBI Official Symbol:
PIWIL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 85, 90 kDa
Observed: 95 kDa
Observed: 95 kDa
Protein Accession #:
AAK69348
Protein GI Number:
14579645
Purification:
PIWI-L1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
Q96J94
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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