CXXC4 Antibody
| Code | Size | Price |
|---|
| PSI-6041-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6041-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
CXXC4 Antibody: IDAX, IDAX, CXXC-type zinc finger protein 4, Inhibition of the Dvl and axin complex protein
Application Note:
CXXC4 antibody can be used for detection of CXXC4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CXXC4 Antibody: CXXC4 is zinc finger protein that binds directly to the PDZ domain of DVL1, a protein involved in the Wnt signaling pathway, through its C-terminal region. CXXC4's interaction with DVL1 competes with DVL1-AXIN binding, although the affinity of DVL1 for CXXC4 is lower than that for AXIN. In mouse fibroblasts, CXXC4 suppressed Wnt3a induction of beta-catenin and inhibited Wnt3a-dependent TCF4 activation, suggesting that CXXC4 acts as a negative regulator of the Wnt signaling pathway and functions between DVL and beta-catenin. Recent reports suggest that decreased CXXC4 expression is associated with kidney cancer metastasis, cell proliferation, reduced apoptosis in response to cancer drugs, and upregulation of genes involved with proliferation, invasion and cell survival, suggesting that CXXC4 plays a critical role in tumor progression in kidney cancer.
Background References:
- Hino S, Kishida S, Michuie T, et al. Inhibition of the Wnt signaling pathway by Idax, a novel Dvl-binding protein. Mol. Cell Biol.2001; 21:330-42.
- Kojima T, Shimazui T, Hinotsu S, et al. Decreased expression of CXXC4 promotes a malignant phenotype in renal cell carcinoma by activating Wnt signaling. Oncogene2009; 28:297-305.
Buffer:
CXXC4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CXXC4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CXXC4.
The immunogen is located within amino acids 50 - 100 of CXXC4.
The immunogen is located within amino acids 50 - 100 of CXXC4.
NCBI Gene ID #:
80319
NCBI Official Name:
CXXC finger protein 4
NCBI Official Symbol:
CXXC4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_079488
Protein GI Number:
13376816
Purification:
CXXC4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q9H2H0
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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