ZIP7 Antibody

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ProSci
Product Code: PSI-6093
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6093-0.02mg0.02mg£150.00
Quantity:
PSI-6093-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZIP7, 6093 (1 μg/mL), ZIP7, 24-029 (4 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZIP7, 6093 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZIP7, 6093 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Western Blot Validation in Mouse Brain Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZIP7, 6093 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 Western Blot Validation in Rat Brain Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZIP7, 6093 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 6 Immunohistochemistry Validation of ZIP7 in Human Brain Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ZIP7 antibody (6093) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 7 Immunofluorescence Validation of ZIP7 in Human Brain Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling ZIP7 with 6093 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZIP7, 6093 (1 μg/mL), ZIP7, 24-029 (4 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZIP7, 6093 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Brain Tissue and 3T3 (Balb) Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZIP7, 6093 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse Brain Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZIP7, 6093 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Western Blot Validation in Rat Brain Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: ZIP7, 6093 (Lane 1: 0.5 μg/mL and Lane 2: 1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 6 Immunohistochemistry Validation of ZIP7 in Human Brain Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-ZIP7 antibody (6093) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunofluorescence Validation of ZIP7 in Human Brain Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human brain tissue labeling ZIP7 with 6093 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Documents

Further Information

Additional Names:
ZIP7 Antibody: KE4, HKE4, ZIP7, RING5, H2-KE4, D6S115E, D6S2244E, Zinc transporter SLC39A7, Histidine-rich membrane protein Ke4
Application Note:
WB: 0.5-1 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ZIP7 Antibody: The zinc transporter ZIP7, also known as SLC39A7, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP7 was initially identified while characterizing genes in the major histocompatibility complex on chromosome 17. ZIP7 mRNA is abundantly and widely expressed and the protein localizes to the Golgi apparatus. It functions to transport intracellular zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 expression is expressed by zinc. ZIP7 has been suggested to act a hub for tyrosine kinase activation and may thus be a potential therapeutic target for diseases such as cancer where prevention of tyrosine kinase activation would be advantageous.
Background References:
  • Dufner-Beattie et al. J. Biol. Chem.2003; 278:50142-50.
  • Eide. Pflugers Arch.2004; 447:796-800.
  • Taylor and Nicohlson. Biochim. Biophys. Acta.2003; 1611:16-30.
  • Lai et al. Genomics1994; 23:338-42.
Buffer:
ZIP7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-ZIP7 antibody (6093) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human ZIP7.

The immunogen is located within amino acids 20-70 of ZIP7.
ISOFORMS:
Human ZIP7 has one isoform (469aa, 50kD). Mouse ZIP7 has one isoform (476aa, 51kD) and Rat ZIP7 also has one isoform (468aa, 50kD). 6093 can detect human, mouse and rat.
NCBI Gene ID #:
7922
NCBI Official Name:
solute carrier family 39 (zinc transporter), member 7
NCBI Official Symbol:
SLC39A7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 50kD

Observed: 50kD
Protein Accession #:
NP_001070984
Protein GI Number:
117553619
Purification:
ZIP7 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
Swissprot #:
Q92504
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar ZIP7 expression profile in human cell lines detected by two independent anti-ZIP7 antibodies that recognize different epitopes, 6093 against N-terminus domain and 24-029 against recombinant fusion protein.  ZIP7 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies. 

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