KLOTHO Antibody

Contact us
ProSci
Product Code: PSI-6107
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6107-0.02mg0.02mg£150.00
Quantity:
PSI-6107-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 6
<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: KLOTHO 6107 (2 μg/mL), KLOTHO, competitor antibody (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
2 / 6
<strong>Figure 2 Western Blot Validation with Recombinant Protein</strong><br> Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO 6107 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 72kD.
3 / 6
<strong>Figure 3 Western Blot Validation in Rat Kidney Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: KLOTHO 6107 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
4 / 6
<strong>Figure 4 Immunohistochemistry Validation of KLOTHO in Mouse Heart Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-KLOTHO antibody (6107) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
5 / 6
<strong>Figure 5 Immunofluorescence Validation of KLOTHO in Mouse Heart Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling KLOTHO with 6107 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
6 / 6
<strong>Figure 6 Immunohistochemistry Validation of KLOTHO in Rat Heart Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-KLOTHO antibody (6107) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
❮❯

<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: KLOTHO 6107 (2 μg/mL), KLOTHO, competitor antibody (4 μg/mL) and beta-actin 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation with Recombinant Protein</strong><br> Loading: 30 ng of human KLOTHO recombinant protein per lane. Antibodies: KLOTHO 6107 (Lane 1: 1 μg/mL, Lane 2: 2 μg/mL and Lane 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 72kD.
<strong>Figure 3 Western Blot Validation in Rat Kidney Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: KLOTHO 6107 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of KLOTHO in Mouse Heart Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-KLOTHO antibody (6107) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunofluorescence Validation of KLOTHO in Mouse Heart Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling KLOTHO with 6107 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
<strong>Figure 6 Immunohistochemistry Validation of KLOTHO in Rat Heart Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-KLOTHO antibody (6107) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
KLOTHO Antibody: Klotho
Application Note:
WB: 1-4 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
KLOTHO Antibody: KLOTHO is the systemic anti-aging hormone within the glycosidase1 superfamily. It encodes a type I membrane protein that is abundant in the kidney and brain. In mice, a deficiency in KLOTHO expression leads to various systemic phenotypes resembling human aging such as arteriosclerosis, osteoporosis, and skin atrophy together with growth retardation, short life-span and infertility. Transgenic mice overexpressing KLOTHO have an extended life span by inhibiting insulin/IGF1 signaling. KLOTHO is involved in the regulation of calcium/phosphorus homeostasis by inhibiting the synthesis of active vitamin D and identified as a potential tumor suppressor.
Background References:
  • Kuro-o et al. Nature1997; 390:45-51.
  • Kurosu et al. Science2005; 309:1829-33.
  • Liu et al. Science 2007; 317:803-6.
  • Wolf et al. Oncogene2008; 27:7094-105.
Buffer:
KLOTHO Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-KLOTHO antibody (6107) was raised against a peptide corresponding to 17 amino acids near the center of human KLOTHO.

The immunogen is located within amino acids 410-460 of KLOTHO.
ISOFORMS:
Human KLOTHO has 2 isoforms, including isoform 1 (1012aa, 116kD) and isoform 2 (549aa, 62kD). Mouse KLOTHO has 2 isoforms, including isoform 1 (1014aa, 116kD) and isoform 2 (550aa, 62kD). Rat KLOTHO has one isoform (1014aa, 117kD). 6107 can detect human, mouse and rat.
NCBI Gene ID #:
9365
NCBI Official Name:
klotho
NCBI Official Symbol:
KL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 116kD

Observed: 116kD
Protein Accession #:
NP_004786
Protein GI Number:
24497614
Purification:
KLOTHO Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
Three isoforms of KLOTHO are known to exist.
Swissprot #:
Q9UEF7
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar KLOTHO expression profile in human and mouse cell lines detected by two independent anti-KLOTHO antibodies that recognize different epitopes, 6107 against central domain and competitor antibody against human recombinant protein.  KLOTHO proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Recombinant Protein Test (Figure 2): Anti-KLOTHO antibodies (6107) detected human KLOTHO recombinant protein at different concentrations.

Related Products

Product NameProduct CodeSupplier 
KLOTHO PeptidePSI-6107PProSci Summary Details