REQUIEM Antibody
| Code | Size | Price |
|---|
| PSI-6109-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6109-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
REQUIEM Antibody: REQ, UBID4, ubi-d4, BAF45D, REQ, Zinc finger protein ubi-d4, Apoptosis response zinc finger protein
Application Note:
REQUIEM antibody can be used for detection of REQUIEM by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
REQUIEM Antibody: Requiem is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It is expressed in numerous tissues in both cytosolic and nuclear regions and likely serves a regulatory role in rapid hematopoietic cell growth and turnover. Requiem has been found to act as an adaptor protein that links the NF-κB and SWI/SNF chromatin remodeling factor and is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors.
Background References:
- Gabig TG, Mantel PL, Rosli R, et al. Requiem: a novel zinc finger gene essential for apoptosis in myeloid cells. J. Biol. Chem.1994; 269:29515-9.
- Gabig TG, Crean CD, Klenk A, et al. Expression and chromosomal localization of the Requiem gene. Mamm. Genome1998; 9:660-5.
- Tando T, Ishizaka A, Watanable H, et al. Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a noncanonical NF-kappaB pathway. J. Biol. Chem.2010; 285:21951-60.
Buffer:
REQUIEM Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (94%)
Immunogen:
REQUIEM antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human Requiem.
The immunogen is located within the last 50 amino acids of REQUIEM.
The immunogen is located within the last 50 amino acids of REQUIEM.
NCBI Gene ID #:
5977
NCBI Official Name:
D4, zinc and double PHD fingers family 2
NCBI Official Symbol:
DPF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAB81203
Protein GI Number:
5454004
Purification:
REQUIEM Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
Requiem antibody is predicted to not cross-react with other DPF protein family members.
Swissprot #:
Q92785
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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