TFF3 Antibody

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ProSci
Product Code: PSI-6113
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6113-0.02mg0.02mg£150.00
Quantity:
PSI-6113-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 6
Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2.5 μg/mL.
3 / 6
Immunofluorescence of TFF3 in Human Small Intestine tissue with TFF3 antibody at 20 μg/mL.
4 / 6
Immunocytochemistry of TFF3 in HeLa cells with TFF3 antibody at 2 μg/ml.
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Immunofluorescence of TFF3 in human small intestine tissue with TFF3 antibody at 20 μg/ml. <br><br>Red: TFF3 Antibody (6113) <br> Blue: DAPI staining
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Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2 μg/ml.
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Western blot analysis of TFF3 in human colon tissue lysate with TFF3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2.5 μg/mL.
Immunofluorescence of TFF3 in Human Small Intestine tissue with TFF3 antibody at 20 μg/mL.
Immunocytochemistry of TFF3 in HeLa cells with TFF3 antibody at 2 μg/ml.
Immunofluorescence of TFF3 in human small intestine tissue with TFF3 antibody at 20 μg/ml. <br><br>Red: TFF3 Antibody (6113) <br> Blue: DAPI staining
Immunohistochemistry of TFF3 in human small intestine tissue with TFF3 antibody at 2 μg/ml.

Documents

Further Information

Additional Names:
TFF3 Antibody: ITF, P1B, TFI, ITF, Trefoil factor 3, Intestinal trefoil factor, hITF
Application Note:
TFF3 antibody can be used for detection of TFF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry at starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TFF3 Antibody: Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. Trefoil peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain and clustered in human chromosome 21q22.3. Trefoil Factor 3 (TFF3) is one of three trefoil peptides secreted by epithelial cells that line mucus membranes in the large and small intestines and lower respiratory tract. TFF3 is mainly active as a disulfide-linked homodimer and may have a role in promoting cell migration in healing processes. It is involved in the maintenance and repair of the intestinal mucosa.
Background References:
  • Podolsky DK, Lynch-Devaney K, Stow JL, et al. Identification of human intestinal trefoil factor. Goblet cell-specific expression of a peptide targeted for apical secretion. J. Biol. Chem. 1993; 268:6694-702.
  • Gott P, Beck S, Machado JC, et al. Human trefoil peptides: genomic structure in 21q22.3 and coordinated expression. Eur. J. Hum. Genet. 1996; 4:308-15.
  • Hernandez C, Santamatilde E, McCreath KJ, et al. Induction of trefoil factor TFF1, TFF2 and TFF3 by hypoxia is mediated by hypoxia inducible factor-1: implications for gastric mucosal healing. Br. J. Pharmacol. 2009; 156:262-72.
  • Thim L. Trefoil peptides: from structure to function. Cell Mol. Life Sci. 1997; 53:888-903.
Buffer:
TFF3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (81%), Pig: (81%)
Immunogen:
TFF3 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human TFF3.

The immunogen is located within the last 50 amino acids of TFF3.
NCBI Gene ID #:
7033
NCBI Official Name:
trefoil factor 3 (intestinal)
NCBI Official Symbol:
TFF3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 10 kDa

Observed: 11 kDa
Protein Accession #:
NP_003217
Protein GI Number:
119629977
Purification:
TFF3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
TFF3 antibody is predicted to not cross-react with other TFF family members.
Swissprot #:
Q07654
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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