N4BP1 Antibody
| Code | Size | Price |
|---|
| PSI-6175-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6175-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
N4BP1 Antibody: KIAA0615, NEDD4-binding protein 1, N4BP1
Application Note:
N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
N4BP1 Antibody: Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.
Background References:
- Murillas R, Simms KS, Hatakeyama S, et al. Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase. J. Biol. Chem. 2002; 277:2897-907.
- Sharma P, Murillas R, Zhang H, et al. N4BP1 is a newly identified nucleolar protein that undergoes SUMO-regulated polyubiquitylation and proteasomal turnover at promyelocytic leukemia nuclear bodies. J. Cell Sci. 2010; 123:1227-34.
- Oberst A, Malatesta M, Aqeilan RI, et al. The Nedd4-binding partner 1 (N4BP1) protein is an inhibitor of the E3 ligase Itch. Proc. Natl. Acad. Sci. USA 2007; 104:11280-5.
Buffer:
N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1.
The immunogen is located within the first 50 amino acids of N4BP1.
The immunogen is located within the first 50 amino acids of N4BP1.
NCBI Gene ID #:
9683
NCBI Official Name:
NEDD4 binding protein 1
NCBI Official Symbol:
N4BP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_694574
Protein GI Number:
48928019
Purification:
N4BP1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.
Swissprot #:
O75113
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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