RBM35A Antibody
| Code | Size | Price |
|---|
| PSI-6185-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6185-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
RBM35A Antibody: RBM35A, RMB35A, RBM35A, Epithelial splicing regulatory protein 1, RNA-binding motif protein 35A
Application Note:
RBM35A antibody can be used for detection of RBM35A by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
RBM35A Antibody: RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.
Background References:
- Warzecha CC, Jiang P, Amirikian K, et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J. 2010; 29:3286-300.
- Warzecha CC, Shen S, Xing Y, et al. The epithelial splicing factors ESRP1 and ESRP2 positively and negatively regulate diverse types of alternative splicing events. RNA Biol. 2009; 6:546-62.
- Leontieva OV and Ionov Y. RNA-binding motif protein 35A is a novel tumor suppressor for colorectal cancer. Cell Cycle 2009; 8:490-7.
- Leontieva OV and Ionov Y. RNA-binding motif protein 35A is a novel tumor suppressor for colorectal cancer. Cell Cycle 2009; 8:490-7.
Buffer:
RBM35A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
RBM35A antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human RBM35A.
The immunogen is located within amino acids 30 - 80 of RBM35A.
The immunogen is located within amino acids 30 - 80 of RBM35A.
NCBI Gene ID #:
54845
NCBI Official Name:
epithelial splicing regulatory protein 1
NCBI Official Symbol:
ESRP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001030087
Protein GI Number:
56790297
Purification:
RBM35A Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
RBM35A antibody is predicted to not cross-react with other RBM35/ESRP family members. At least five isoforms of RBM35A are known to exist; this antibody will detect all five.
Swissprot #:
Q6NXG1
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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