RABEX5 Antibody
| Code | Size | Price |
|---|
| PSI-6225-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6225-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
RABEX5 Antibody: RAP1, RABEX5, rabex-5, RAP1
Application Note:
RABEX5 antibody can be used for detection of RABEX5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
RABEX5 Antibody: RABEX5 is a guanine nucleotide exchange factor (GEF) that forms a complex with rabaptin-5 and specifically activates Rab5, a small GTPase that plays an essential role in endocytic traffic. RABEX-5 is thought to also interact with another member of the Rab family, Rab22, which targets RABEX5 to early endosomes, allowing it to activate Rab5 and thereby promote Rab5-mediated endosome fusion.
Background References:
- Horiuchi H, Lippe R, McBride HM, et al. A novel Rab5 GDP/GTP exchange factor complexed to Rabaptin-5 links nucleotide exchange to effector recruitment and function. Cell 1997; 90:1149-59.
- Zhu H, Liang Z, and Li G. Rabex-5 is a Rab22 effector and mediates a Rab22-Rab5 signaling cascade in endocytosis. Mol. Biol. Cell 2009; 20:4720-9.
Buffer:
RABEX5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
RABEX5 antibody was raised against a 19 amino acid synthetic peptide near the center terminus of human RABEX5.
The immunogen is located within amino acids 430 - 480 of RABEX5.
The immunogen is located within amino acids 430 - 480 of RABEX5.
NCBI Gene ID #:
27342
NCBI Official Name:
RAB guanine nucleotide exchange factor (GEF) 1
NCBI Official Symbol:
RABGEF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055319
Protein GI Number:
7657496
Purification:
RABEX5 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
At least four isoforms of RABEX5 are known to exist; this antibody will recognize all four. RABEX5 antibody is predicted to not cross-react with other LRRFIP family members.
Swissprot #:
Q9UJ41
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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