TM4SF1 Antibody

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ProSci
Product Code: PSI-6229
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6229-0.02mg0.02mg£150.00
Quantity:
PSI-6229-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TM4SF1 in human lung tissue lysate with TM4SF1 antibody at (A) 0.5 and (B) 1 μg/mL.
2 / 6
Immunohistochemistry of TM4SF1 in human lung tissue with TM4SF1 antibody at 5 μg/mL.
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Immunofluorescence of TM4SF1 in human lung tissue with TM4SF1 antibody at 20 μg/mL.
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Immunohistochemistry of TM4SF1 in mouse lung tissue with TM4SF1 antibody at 5 μg/mL.
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Immunofluorescence of TM4SF1 in mouse lung tissue with TM4SF1 antibody at 20 μg/mL.<br><br>Red: TM4SF1 Antibody (6229) <br> Blue: DAPI staining
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Immunohistochemistry of TM4SF1 in mouse lung tissue with TM4SF1 antibody at 2 μg/mL.
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Western blot analysis of TM4SF1 in human lung tissue lysate with TM4SF1 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of TM4SF1 in human lung tissue with TM4SF1 antibody at 5 μg/mL.
Immunofluorescence of TM4SF1 in human lung tissue with TM4SF1 antibody at 20 μg/mL.
Immunohistochemistry of TM4SF1 in mouse lung tissue with TM4SF1 antibody at 5 μg/mL.
Immunofluorescence of TM4SF1 in mouse lung tissue with TM4SF1 antibody at 20 μg/mL.<br><br>Red: TM4SF1 Antibody (6229) <br> Blue: DAPI staining
Immunohistochemistry of TM4SF1 in mouse lung tissue with TM4SF1 antibody at 2 μg/mL.

Documents

Further Information

Additional Names:
TM4SF1 Antibody: L6, H-L6, M3S1, TAAL6, Transmembrane 4 L6 family member 1, Membrane component chromosome 3 surface marker 1
Application Note:
TM4SF1 antibody can be used for detection of TM4SF1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
TM4SF1 Antibody: TM4SF1 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains and mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. TM4SF1 is a cell surface antigen and is highly expressed in the vascular endothelium of several human cancers. Knockdown experiments of TM4SF1 prevented filopodia formation, inhibited cell mobility, blocked cytokinesis, and inhibited maturation of VEGF-A-induced angiogenesis, suggesting that TM4SF1 may be an attractive target for anti-angiogenesis therapy.
Background References:
  • Marken JS, Schieven GL, Hellstrom I, et al. Cloning and expression of the tumor-associated antigen L6. Proc. Natl. Acad. Sci. USA 1992; 89:3503-7
  • Romanska HM and Berditchevski F. Tetraspanins in human epithelial malignancies. J. Pathol. 2011; 223:4-14
  • Shih SC, Zukauskas A, Li D, et al. The L6 TM4SF1 is critical for endothelial cell function and tumor angiogenesis. Cancer Res. 2009; 69:3272-7.
Buffer:
TM4SF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TM4SF1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human TM4SF1.

The immunogen is located within the first 50 amino acids of TM4SF1.
NCBI Gene ID #:
4071
NCBI Official Name:
transmembrane 4 L six family member 1
NCBI Official Symbol:
TM4SF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055035
Protein GI Number:
21265101
Purification:
TM4SF1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least three isoforms of TM4SF1 are known to exist; this antibody will recognize only the two largest isoforms. TM4SF1 antibody is predicted to not cross-react with other tetraspanin proteins.
Swissprot #:
P30408
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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