LIF Antibody

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ProSci
Product Code: PSI-6245
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6245-0.02mg0.02mg£150.00
Quantity:
PSI-6245-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation in Mouse 3T3 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LIF 6245, (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LIF 6245, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation with Recombinant Protein</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LIF 6245, (1: 1 μg/mL, 2: 2μg/mL, 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Western Blot Validation in Mouse Cell Lines and Tissue</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LIF 6245, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 5 Western Blot Validation in Rat Lung</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LIF 6245, (1: 1 μg/mL, 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 6 Immunofluorescence Validation of LIF in Mouse 3T3 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 cells labeling LIF with 6245 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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<strong>Figure 1 Western Blot Validation in Mouse 3T3 Cell Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LIF 6245, (A: 1 μg/mL, B: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LIF 6245, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation with Recombinant Protein</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LIF 6245, (1: 1 μg/mL, 2: 2μg/mL, 3: 4 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse Cell Lines and Tissue</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LIF 6245, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Western Blot Validation in Rat Lung</strong><br> Loading: 15 μg of lysates per lane. Antibodies: LIF 6245, (1: 1 μg/mL, 2: 2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 6 Immunofluorescence Validation of LIF in Mouse 3T3 Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed 3T3 cells labeling LIF with 6245 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Documents

Further Information

Additional Names:
LIF Antibody: CDF, DIA, HILDA, MLPLI, Leukemia inhibitory factor, Differentiation-stimulating factor, LIF
Application Note:
WB: 1-4 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
LIF Antibody: LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF was initially recognized by its ability to induce terminal differentiation of myeloid leukemic cells. It is a member of the IL-6 cytokine superfamily and can be highly glycosylated. LIF signaling is transduced through the LIF-R/gp130 receptor complex, leading to the phosphorylation and activation of the JAK/STAT pathway. Recent evidence shows that LIF inhibits cardiomyogenesis in embryonic stem cells via STAT3 activation.
Background References:
  • Trouillas et al. Cytokine Netw. 2009; 20:51-62.
  • Hilton et al. Anal. Biochem. 1988; 173:359-67.
  • Schemlzer et al. Protein Expr. Purif. 1990; 1:54-62.
Buffer:
LIF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
Anti-LIF antibody (6245) was raised against a peptide corresponding to 16 amino acids near the center of human LIF.

The immunogen is located within amino acids 50-100 of LIF.
ISOFORMS:
Human LIF has 2 isoforms, including isoform 1 (202aa, 22kD) and isoform 2 (88aa, 9.6kD). Mouse LIF has 1 isoform (203aa, 22.3kD and Rat LIF also has 1 isoform (202aa, 22.1kD). 6245 can detect human isoform1 as well as mouse and rat LIF.
NCBI Gene ID #:
3976
NCBI Official Name:
leukemia inhibitory factor
NCBI Official Symbol:
LIF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 22kD

Observed: 26kD (Post-modifications: N-linked Glycosylations)
Protein Accession #:
NP_002300
Protein GI Number:
4504991
Purification:
LIF Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
At least two isoforms of LIF are known to exist.
Swissprot #:
P15018
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Recombinant Protein Test (Figure 3): Anit-LIF antibodies (6245) detected human LIF recombinant protein at different concentrations.

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