DYRK2 Antibody

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ProSci
Product Code: PSI-6257
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6257-0.02mg0.02mg£150.00
Quantity:
PSI-6257-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of DYRK2 in 293 cell lysate with DYRK2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of DYRK2 in 293 cells with DYRK2 antibody at 10 μg/mL.
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Immunofluorescence of DYRK2 in 293 cells with DYRK2 antibody at 20 μg/mL.
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Western blot analysis of DYRK2 in 293 cell lysate with DYRK2 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of DYRK2 in 293 cells with DYRK2 antibody at 10 μg/mL.
Immunofluorescence of DYRK2 in 293 cells with DYRK2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
DYRK2 Antibody: Dual specificity tyrosine-phosphorylation-regulated kinase 2
Application Note:
DYRK2 antibody can be used for detection of DYRK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
DYRK2 Antibody: DYRK2 is a member of the dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family that is thought to be involved in cellular proliferation and apoptosis. Under normal conditions, nuclear but not cytoplasmic DYRK2 is ubiquitinated by MDM2, leading to its constitutive degradation. However, upon exposure to genotoxic stress, ATM phosphorylates DYRK2, leading to its dissociation from MDM2 and its phosphorylation of p53, thereby inducing apoptosis. Recent evidence also suggests that DYRK2 may serve as a scaffold that facilitates assembly of an E3 ubiquitin ligase.
Background References:
  • Becker W, Weber Y, Wetzel K, et al. Sequence characteristics, subcellular localization, and substrate specificity of DRYK-related kinases, a novel family of dual specificity protein kinases. J. Biol. Chem. 1998; 273:25893-902.
  • Yoshida K. Role for DYRK family kinases on regulation of apoptosis. Biochem. Pharmacol. 2008; 76:1389-94.
  • Taira N, Yamamoto H, Yamaguchi T, et al. ATM augments nuclear stabilization of DYRK2 by inhibiting MDM2 in the apoptotic response to DNA damage J. Biol. Chem. 2010; 285:4909-19.
Buffer:
DYRK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (89%), Mouse: (89%)
Immunogen:
DYRK2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DYRK2.

The immunogen is located within amino acids 520 - 570 of DYRK2.
NCBI Gene ID #:
8445
NCBI Official Name:
dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2
NCBI Official Symbol:
DYRK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_006473
Protein GI Number:
4503427
Purification:
DYRK2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
Two isoforms of DYRK2 are known to exist; this antibody will recognize both isoforms. DYRK2 antibody will not cross-react with other DYRK family members.
Swissprot #:
Q92630
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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