ATP2C1 Antibody
| Code | Size | Price |
|---|
| PSI-6287-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6287-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ATP2C1 Antibody: HHD, BCPM, PMR1, SPCA1, hSPCA1, ATP2C1A, KIAA1347, PMR1L, HUSSY-28, Calcium-transporting ATPase type 2C member 1, ATPase 2C1
Application Note:
ATP2C1 antibody can be used for detection of ATP2C1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
ATP2C1 Antibody: ATP2C1, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder characterized by persistent blisters and erosions of the skin. Unlike the related protein ATP2C2, ATP2C1 is ubiquitously expressed and displays a lower maximal turnover rate for overall Ca2+-ATPase reaction and a higher apparent affinity for cytosolic Ca2+ activation of phosphorylation. Recent evidence suggests that ATP2C1 is a key regulator of insulin-like growth factor receptor (IGF1R) processing in tumor progression in basal breast cancers.
Background References:
- Hu Z, Bonifas JM, Beech J, et al. Mutations in ATP2C1, encoding a calcium pump, cause Hailey-Hailey disease. Nat. Genet. 2000; 24:61-5
- Dode L, Andersen JP, Vanoevelen J, et al. Dissection of the functional differences between human secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1 and 2 isoenzymes by steady-state and transient kinetic analyses. J. Biol. Chem. 281:3182-9.
- Grice DM, Vetter I, Faddy HM, et al. Golgi calcium pump secretory pathway calcium ATPase 1 (SPCA1) is a key regulator of insulin-like growth factor receptor (IGF1R) processing in the basal-like breast cancer cell line MDA-MB-231. J. Biol. Chem. 2010; 285:37458-66
Buffer:
ATP2C1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%), Rat: (86%)
Immunogen:
ATP2C1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ATP2C1.
The immunogen is located within the last 50 amino acids of ATP2C1.
The immunogen is located within the last 50 amino acids of ATP2C1.
NCBI Gene ID #:
27032
NCBI Official Name:
ATPase, Ca++ transporting, type 2C, member 1
NCBI Official Symbol:
ATP2C1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001001486
Protein GI Number:
312836765
Purification:
ATP2C1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least four isoforms of ATP2C1 are known to exist; this antibody will recognize only the three longest isoforms. ATP2C1 antibody will not cross-react with ATP2C2.
Swissprot #:
P98194
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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