ATP2C2 Antibody

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ProSci
Product Code: PSI-6289
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6289-0.02mg0.02mg£150.00
Quantity:
PSI-6289-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of ATP2C2 in 3T3 cell lysate with ATP2C2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
2 / 2
Immunofluorescence of ATP2C2 in 3T3 cells with ATP2C2 antibody at 20 μg/mL.
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Western blot analysis of ATP2C2 in 3T3 cell lysate with ATP2C2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of ATP2C2 in 3T3 cells with ATP2C2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
ATP2C2 Antibody: SPCA2, KIAA0703, SPCA2, Calcium-transporting ATPase type 2C member 2, ATPase 2C2
Application Note:
ATP2C2 antibody can be used for detection of ATP2C2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
ATP2C2 Antibody: ATP2C2, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 2, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in the related gene ATP2C1 cause Hailey-Hailey disease, for which ATP2C2 does not compensate, suggesting that ATP2C2 plays other physiological roles. Unlike ATP2C1, ATP2C2 has a much more restricted expression pattern and displays a higher maximal turnover rate for overall Ca2+-ATPase reaction and a lower apparent affinity for cytosolic Ca2+ activation of phosphorylation. Overexpression of ATP2C2 in mammary tumors result a Ca2+ influx via the store-operated Ca2+ channel ORAI1 and independent of the STIM1 and STIM2 sensors.
Background References:
  • Xiang M, Mohamalawari D, and Rao R. A novel isoform of the secretory pathway Ca2+,Mn(2+)-ATPase, hSPCA2, has unusual properties and is expressed in the brain. J. Biol. Chem. 2005; 280:11608-14.
  • Hu Z, Bonifas JM, Beech J, et al. Mutations in ATP2C1, encoding a calcium pump, cause Hailey-Hailey disease. Nat. Genet. 2000; 24:61-5
  • Dode L, Andersen JP, Vanoevelen J, et al. Dissection of the functional differences between human secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1 and 2 isoenzymes by steady-state and transient kinetic analyses. J. Biol. Chem. 281:3182-9.
Buffer:
ATP2C2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ATP2C2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP2C2.

The immunogen is located within the first 50 amino acids of ATP2C2.
NCBI Gene ID #:
9914
NCBI Official Name:
ATPase, Ca++ transporting, type 2C, member 2
NCBI Official Symbol:
ATP2C2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055676
Protein GI Number:
118498343
Purification:
ATP2C2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
At least three isoforms of ATP2C2 are known to exist. ATP2C2 antibody will not cross-react with ATP2C1
Swissprot #:
O75185
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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