PRR5L Antibody
| Code | Size | Price |
|---|
| PSI-6293-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6293-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PRR5L Antibody: PROTOR2, PROTOR2, Proline-rich protein 5-like, Protein observed with Rictor-2, Protor-2
Application Note:
PRR5L antibody can be used for detection of PRR5L by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
PRR5L Antibody: Proline-rich protein 5-like (PRR5L), also known as Protor-2, is structurally similar to PRR5 and also binds to the mTOR complex 2 (mTORC2). PRR5L is not required for mTORC2 integrity or kinase activity, but dissociates from mTORC2 upon knockdown of tuberous sclerosis complex 1 (TSC1) and TSC2. Knockdown of PRR5L reduced apoptosis in mTORC2 deficient cells as well as cells where mTOR was active, suggesting that mTORC2-dissociated PRR5L may promote apoptosis, and thus play a role in the balance between cell growth and cell death.
Background References:
- Thedieck K, Polak P, Kim ML, et al. 2007. PRAS40 and PRR5-like protein are new mTOR interactors that regulate apoptosis. PLoS One 2007; 2:e1217.
- Pearce LR, Huang X, Boudeau J, et al. Identification of Protor as a novel Rictor-binding component of mTOR complex-2. Biochem. J. 2007; 405:513-22.
Buffer:
PRR5L Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (88%)
Immunogen:
PRR5L antibody was raised against a 17 amino acid synthetic peptide near the center of human PRR5L.
The immunogen is located within amino acids 220 - 270 of PRR5L.
The immunogen is located within amino acids 220 - 270 of PRR5L.
NCBI Gene ID #:
79899
NCBI Official Name:
proline rich 5 like
NCBI Official Symbol:
PRR5L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001153639
Protein GI Number:
237757351
Purification:
PRR5L Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
At least three isoforms of PRR5L are known to exist; this antibody will recognize only isoforms a and b. PRR5L antibody will not cross-react with PPR5.
Swissprot #:
Q6MZQ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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