ROCK2 Antibody

Contact us
ProSci
Product Code: PSI-6297
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6297-0.02mg0.02mg£150.00
Quantity:
PSI-6297-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 μg/mL
2 / 3
Immunohistochemistry of ROCK2 in mouse brain tissue with ROCK2 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 μg/mL.
❮❯

Western blot analysis of ROCK2 in mouse brain tissue lysate with ROCK2 antibody at (A) 1 and (B) 2 μg/mL
Immunohistochemistry of ROCK2 in mouse brain tissue with ROCK2 antibody at 5 μg/mL.
Immunofluorescence of ROCK2 in mouse brain tissue with ROCK2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
ROCK2 Antibody: ROCK-II, KIAA0619, Rho-associated protein kinase 2, Rho kinase 2, ROCK-II
Application Note:
ROCK2 antibody can be used for detection of ROCK2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
ROCK2 Antibody: ROCK2 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK2 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK2, like its isozyme ROCK1, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.
Background References:
  • Leung T, Chen XQ, Manser E, et al. The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton. Mol. Cell Biol. 1996; 16:5313-27.
  • Takahashi N, Tuiki H, Saya H, et al. Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24. Genomics 1999; 55:235-7
  • Ishizaki T, Maekawa M, Fujisawa K, et al. The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase. EMBO J. 1996; 15:1885-93.
Buffer:
ROCK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%)
Immunogen:
ROCK2 antibody was raised against a 15 amino acid synthetic peptide near the center of human ROCK2.

The immunogen is located within amino acids 920 - 970 of ROCK2.
NCBI Gene ID #:
9475
NCBI Official Name:
Rho-associated, coiled-coil containing protein kinase 2
NCBI Official Symbol:
ROCK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 153 kDa

Observed: 160 kDa
Protein Accession #:
NP_004841
Protein GI Number:
41872583
Purification:
ROCK2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
Three isoforms of ROCK2 are known to exist; this antibody recognizes all three isoforms. ROCK2 antibody is predicted to not cross-react with other ROCK protein family members.
Swissprot #:
O75116
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier 
ROCK2 PeptidePSI-6297PProSci Summary Details