EZH1 Antibody

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ProSci
Product Code: PSI-6261
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6261-0.02mg0.02mg£150.00
Quantity:
PSI-6261-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of EZH1 in mouse lung tissue lysate with EZH1 antibody at (A) 1 and (B) 2 μg/mL
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Immunohistochemistry of EZH1 in human lung tissue with EZH1 antibody at 5 μg/mL.
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Immunofluorescence of EZH1 in human lung tissue with EZH1 antibody at 20 μg/mL.
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Western blot analysis of EZH1 in mouse lung tissue lysate with EZH1 antibody at (A) 1 and (B) 2 μg/mL
Immunohistochemistry of EZH1 in human lung tissue with EZH1 antibody at 5 μg/mL.
Immunofluorescence of EZH1 in human lung tissue with EZH1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
EZH1 Antibody: KMT6B, KIAA0388, Histone-lysine N-methyltransferase EZH1, ENX-2
Application Note:
EZH1 antibody can be used for detection of EZH1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
EZH1 Antibody: EZH1 was initially identified as a homolog of the drosophila Enhancer of Zeste through transcription mapping of chromosome 17q21. Both EZH1 and the related protein EZH2 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair.
Background References:
  • Abel KJ, Brody LC, Valdes JM, et al. Characterization of EZH1, a human homolog of Drosophila Enhancer of zeste near BRCA1. Genomics 1996; 37:161-71
  • Margueron R, Li G, Sarma K, et al. Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms. Mol. Cell 2008; 32:503-18.
  • Shen X, Liu Y, Hsu YJ, et al. EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency. Mol. Cell 2008; 32:491-502
  • Ezhkova E, Lien WH, Stokes N, et al. EZH1 and EZH2 cogovern histone H3K27 trimethylation and are essential for hair follicle homeostasis and wound repair. Genes Dev. 2011; 25:485-98
Buffer:
EZH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
EZH1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EZH1.

The immunogen is located within amino acids 180 - 230 of EZH1.
NCBI Gene ID #:
2145
NCBI Official Name:
enhancer of zeste homolog 1
NCBI Official Symbol:
EZH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAC50778
Protein GI Number:
19923202
Purification:
EZH1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
Multiple isoforms of EZH1 are known to exist. EZH1 antibody is predicted to not cross-react with EZH2
Swissprot #:
Q92800
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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