EZH2 Antibody

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ProSci
Product Code: PSI-6263
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6263-0.02mg0.02mg£150.00
Quantity:
PSI-6263-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of EZH2 in 293 cell lysate with EZH2 antibody at 1 μg/mL.
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Immunocytochemistry of EZH2 in 293 cells with EZH2 antibody at 10 μg/mL.
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Immunofluorescence of EZH2 in 293 cells with EZH2 antibody at 20 μg/mL.
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Western blot analysis of EZH2 in 293 cell lysate with EZH2 antibody at 1 μg/mL.
Immunocytochemistry of EZH2 in 293 cells with EZH2 antibody at 10 μg/mL.
Immunofluorescence of EZH2 in 293 cells with EZH2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
EZH2 Antibody: WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A, Histone-lysine N-methyltransferase EZH2
Application Note:
EZH2 antibody can be used for detection of EZH2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
EZH2 Antibody: EZH2 was initially identified as a homolog of the drosophila Enhancer of Zeste through exon trap screening of chromosome 21. Both EZH2 and the related protein EZH1 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair. Overexpression of EZH2 has been reported as a marker for advanced and metastatic cancers.
Background References:
  • Chen H, Rossier C, and Antonarakis SE. Cloning of a human homolog of the Drosophila enhancer of zeste gene (EZH2) that maps to chromosome 21q22.2. Genomics 1996; 38:30-7
  • Margueron R, Li G, Sarma K, et al. Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms. Mol. Cell 2008; 32:503-18.
  • Shen X, Liu Y, Hsu YJ, et al. EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency. Mol. Cell 2008; 32:491-502.
  • Ezhkova E, Lien WH, Stokes N, et al. EZH1 and EZH2 cogovern histone H3K27 trimethylation and are essential for hair follicle homeostasis and wound repair. Genes Dev. 2011; 25:485-98.
Buffer:
EZH2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
EZH2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human EZH2.

The immunogen is located within amino acids 190 - 240 of EZH2.
NCBI Gene ID #:
2146
NCBI Official Name:
enhancer of zeste homolog 2
NCBI Official Symbol:
EZH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
AAH10858
Protein GI Number:
21361095
Purification:
EZH2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
Multiple isoforms of EZH2 are known to exist. EZH2 antibody is predicted to not cross-react with EZH1.
Swissprot #:
Q15910
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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