SIK1 Antibody
| Code | Size | Price |
|---|
| PSI-6299-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6299-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SIK1 Antibody: MSK, SIK, SNF1LK, Salt-inducible kinase 1, SIK-1
Application Note:
SIK1 antibody can be used for detection of SIK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
SIK1 Antibody: Salt-inducible kinase 1 (SIK1), also known as SNF1LK or MSK, plays a role in histone modification and G2/M cell cycle regulation. It is a 783 amino acid protein that contains one UBA domain and belongs to the Ser/Thr protein kinase family (AMPK subfamily). Localized to both the nucleus and the cytoplasm, SIK1 is a class II HDAC kinase that uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation.
Background References:
- Stephenson A, Huang GY, Nguyen NT, et al. SNF1LK encodes a protein kinase that may function in cell cycle regulation. Genomics 2004; 83:1105-15
- Ruiz JC, Conlon FL, and Robertson EJ. Identification of novel protein kinases expressed in the myocardium of the developing mouse heart. Mech. Dev. 1994; 48:153-64
- Lizcano JM, Goransson O, Toth R, et al. LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. EMBO J. 2004; 23:833-43.
- Sjöström M, Senstrom K, Eneling K, et al. SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process. Proc. Natl. Acad. Sci. USA 2007; 104:16922-7.
Buffer:
SIK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (73%), Mouse: (73%)
Immunogen:
SIK1 antibody was raised against an 18 amino acid synthetic peptide near the center of human SIK1.
The immunogen is located within amino acids 340 - 390 of SIK1.
The immunogen is located within amino acids 340 - 390 of SIK1.
NCBI Gene ID #:
150094
NCBI Official Name:
salt-inducible kinase 1
NCBI Official Symbol:
SIK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_775490
Protein GI Number:
116256471
Purification:
SIK1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
Two isoforms of SIK1 are known to exist; this antibody will detect both isoforms. SIK1 antibody is predicted to not cross-react with other SIK protein family members.
Swissprot #:
P57059
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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