SIK2 Antibody

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ProSci
Product Code: PSI-6301
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6301-0.02mg0.02mg£150.00
Quantity:
PSI-6301-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of SIK2 in SW480 cell lysate with SIK2 antibody at 1 μg/mL.
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Immunocytochemistry of SIK2 in SW480 cells with SIK2 antibody at 2.5 μg/ml.
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Immunofluorescence of SIK2 in SW480 cells with SIK2 antibody at 5 μg/ml.
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Western blot analysis of SIK2 in SW480 cell lysate with SIK2 antibody at 1 μg/mL.
Immunocytochemistry of SIK2 in SW480 cells with SIK2 antibody at 2.5 μg/ml.
Immunofluorescence of SIK2 in SW480 cells with SIK2 antibody at 5 μg/ml.

Documents

Further Information

Additional Names:
SIK2 Antibody: QIK, SNF1LK2, LOH11CR1I, KIAA0781, QIK, Qin-induced kinase, SIK-2
Application Note:
SIK2 antibody can be used for detection of SIK2 by Western blot at 1 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SIK2 Antibody: Salt-inducible kinase 2 (SIK2), like its closely related homolog SIK1, belongs AMPK subfamily of the Ser/Thr protein kinase family and negatively regulates CRE-binding protein (CREB) activity by phosphorylating the CREB-specific coactivator transducer of regulated CREB activity (TORC). SIK2 is thought to be part of a signaling cascade that regulates the expression and activity of the insulin-induced genes PGC-1 alpha and UCP-1 in brown adipocytes, impairment of which has been implicated in obesity and insulin resistance in human and animal models. SIK2 has also been reported as a key regulator for neuronal survival after ischemia, suppressing CREB-mediated gene expression after oxygen-glucose deprivation.
Background References:
  • Horike N, Takemori H, Katoh Y, et al. Adipose-specific expression, phosphorylation of Ser794 in insulin receptor substrate-1, and activation in diabetic animals of salt-inducible kinase-2. J. Biol. Chem. 2003; 278:18440-7.
  • Screaton RA, Conkright MD, Katoh Y, et al. The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector. Cell 2004; 119:61-74.
  • Muraoka M, Fukushima A, Viengchareun S, et al. Involvement of SIK2/TORC2 signaling cascade in the regulation of insulin-induced PGC-1alpha and UCP-1 gene expression in brown adipocytes. Am. J. Physiol. Endocrinol. Metab. 2009; 296:E1430-9.
  • Sasaki T, Takemori H, Yagita Y, et al. SIK2 is a key regulator for neuronal survival after ischemia via TORC1-CREB. Neuron 2011; 69:106-19.
Buffer:
SIK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SIK2 antibody was raised against a 16 amino acid synthetic peptide near the center of human SIK2.

The immunogen is located within amino acids 270 - 320 of SIK2.
NCBI Gene ID #:
23235
NCBI Official Name:
salt-inducible kinase 2
NCBI Official Symbol:
SIK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
EAW67148
Protein GI Number:
38569460
Purification:
SIK2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
SIK2 antibody is predicted to not cross-react with other SIK protein family members.
Swissprot #:
Q9H0K1
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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