SGK1 Antibody
| Code | Size | Price |
|---|
| PSI-6319-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6319-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
SGK1 Antibody: SGK, SGK
Application Note:
SGK1 antibody can be used for detection of SGK1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SGK1 Antibody: Serum and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases that plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. SGK1 functions as a regulator of cell survival and ion channels and transporters such as ENaC or KCNA3/Kv1.3. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.
Background References:
- Webster MK, Goya L, Ge Y, et al. Characterization of sgk, a novel member of the serine/threonine protein kinase gene family which is transcriptionally induced by glucocorticoids and serum. Mol. Cell Biol. 1993; 13:2031-40.
- Leong ML, Maiyar AC, Kim B, et al. Expression of the serum- and glucocorticoid-inducible protein kinase, Sgk, is a cell survival response to multiple types of environmental stress stimuli in mammary epithelial cells. J. Biol. Chem. 2003; 278:5871-82.
- Firestone GL, Giampaolo JR, O'Keeffe BA, et al. Stimulus-dependent regulation of serum and glucocorticoid inducible protein kinase (SGK) transcription, subcellular localization and enzymatic activity. Cell. Physiol. Biochem. 2003; 13:1-12.
- Brunet A, Park J, Tran H, et al. Protein kinase SGK mediates survival signals by phosphorylating the forkhead transcription factor FKHRL1 (FOXO3a). Mol. Cell Biol. 2001; 21:952-65.
Buffer:
SGK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (89%), Chicken: (84%), Rabbit: (89%)
Immunogen:
SGK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SGK1.
The immunogen is located within amino acids 370 - 420 of SGK1.
The immunogen is located within amino acids 370 - 420 of SGK1.
NCBI Gene ID #:
6446
NCBI Official Name:
serum/glucocorticoid regulated kinase 1
NCBI Official Symbol:
SGK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005618
Protein GI Number:
25168263
Purification:
SGK1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
SGK1 antibody is predicted to not cross-react with other SGK protein family members. At least five isoforms are known to exist
Swissprot #:
O00141
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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