TYW1 Antibody
| Code | Size | Price |
|---|
| PSI-6373-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6373-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TYW1 Antibody: TYW1A, RSAFD1, YPL207W, S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase, Radical S-adenosyl methionine and flavodoxin domain-containing protein 1
Application Note:
TYW1 antibody can be used for detection of TYW1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
TYW1 Antibody: TYW1, also known as TYW1A, RSAFD1 or YPL207W, is a 732 amino acid protein containing one flavodoxin-like domain that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW1 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation. TYW1 binds to one 4Fe-4S cluster and is located on human chromosome 7. Defects in some of the genes on chromosome 7 have been linked to Osteogenesis imperfecta, Williams-Beuren syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome, suggesting that TYW1 may play a role in these conditions
Background References:
- Noma A and Suzuki T. Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp. Ser. (Oxf) 2006; 65-6
- Noma A, Kirino Y, Ikeuchi Y, et al. Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J. 2006; 25:2142-54
- Hillier LW, Fulton RS, Fulton LA, et al. The DNA sequence of human chromosome 7. Nature 2003; 424:157-64.
- Goto-Ito S, Ishii R, Ito T, et al. Structure of an archaeal TYW1, the enzyme catalyzing the second step of wye-base biosynthesis. Acta Crystallogr. D Biol. Crystallogr. 2007; 63:1059-68
Buffer:
TYW1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (89%)
Immunogen:
TYW1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human TYW1.
The immunogen is located within amino acids 520 - 570 of TYW1.
The immunogen is located within amino acids 520 - 570 of TYW1.
NCBI Gene ID #:
55253
NCBI Official Name:
tRNA-yW synthesizing protein 1 homolog (S. cerevisiae)
NCBI Official Symbol:
TYW1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_060734
Protein GI Number:
50726981
Purification:
TYW1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
TYW1 antibody is predicted to not cross-react with other TYW protein family members. At least three isoforms of TYW1 are known to exist; this antibody will only detect the two larger isoforms.
Swissprot #:
Q9NV66
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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