TYW4 Antibody
| Code | Size | Price |
|---|
| PSI-6379-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6379-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
TYW4 Antibody: PPM2, TYW4, KIAA0547, tRNA wybutosine-synthesizing protein 4, Leucine carboxyl methyltransferase 2, tRNA yW-synthesizing protein 4
Application Note:
TYW4 antibody can be used for detection of TYW4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
TYW4 Antibody: TYW4 is an enzyme that participates in the wybutosine-tRNA (Phe) biosynthesis pathway. Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TYW4 is involved in a multistep enzymatic reaction that stabilizes codon-anticodon base-pairing during the ribosomal decoding process, thereby ensuring correct translation.
Background References:
- Noma A and Suzuki T. Ribonucleome analysis identified enzyme genes responsible for wybutosine synthesis. Nucleic Acids Symp. Ser. (Oxf) 2006; 65-6.
- Noma A, Kirino Y, Ikeuchi Y, et al. Biosynthesis of wybutosine, a hyper-modified nucleoside in eukaryotic phenylalanine tRNA. EMBO J. 2006; 25:2142-54.
Buffer:
TYW4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TYW4 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human TYW4.
The immunogen is located within the first 50 amino acids of TYW4.
The immunogen is located within the first 50 amino acids of TYW4.
NCBI Gene ID #:
9836
NCBI Official Name:
leucine carboxyl methyltransferase 2
NCBI Official Symbol:
LCMT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_055608
Protein GI Number:
25188199
Purification:
TYW4 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
TYW4 antibody is predicted to not cross-react with other TYW protein family members. At least two isoforms of TYW4 are known to exist; this antibody will detect only the long isoform.
Swissprot #:
O60294
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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