SPRYD3 Antibody

ProSci
Product Code: PSI-6405
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6405-0.02mg0.02mg£150.00
Quantity:
PSI-6405-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of SPRYD3 in human brain tissue lysate with SPRYD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
2 / 3
Immunohistochemistry of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 20 μg/mL.

Western blot analysis of SPRYD3 in human brain tissue lysate with SPRYD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunohistochemistry of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 5 μg/mL.
Immunofluorescence of SPRYD3 in mouse brain tissue with SPRYD3 antibody at 20 μg/mL.

Further Information

Additional Names:
SPRYD3 Antibody: SPRY domain-containing protein 3
Application Note:
SPRYD3 antibody can be used for detection of SPRYD3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
SPRYD3 Antibody: The SPRY domain-containing protein 3 (SPRYD3) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. Little is known of the function of the SPRYD3 protein.
Background References:
  • Tae H, Casarotto MG, and Dulhunty AF. Ubiquitous SPRY domains and their role in the skeletal type ryanodine receptor. Eur. Biophys. J. 2009; 39:51-9.
Buffer:
SPRYD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SPRYD3 antibody was raised against a 16 amino acid synthetic peptide near the center of human SPRYD3.

The immunogen is located within amino acids 260 - 310 of SPRYD3.
NCBI Gene ID #:
84926
NCBI Official Name:
SPRY domain containing 3
NCBI Official Symbol:
SPRYD3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_116229
Protein GI Number:
14249554
Purification:
SPRYD3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
SPRYD3 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD3 are known to exist.
Swissprot #:
Q8NCJ5
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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