KCNK12 Antibody
| Code | Size | Price |
|---|
| PSI-6425-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6425-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
KCNK12 Antibody: THIK2, THIK-2, K2p12.1, Potassium channel subfamily K member 12, Tandem pore domain halothane-inhibited potassium channel 2
Application Note:
KCNK12 antibody can be used for detection of KCNK12 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
KCNK12 Antibody: KCNK13 and KCNK12 (also known as THIK1 and 2) are the first two members of a novel two pore-forming P domains K+ channels subfamily. The pore loop domain, a highly conserved region common to all potassium channels, is involved in determining potassium ion selectivity. Members of this family are all characterized by four transmembrane domains and may function to help influence the resting membrane potential of cells. KCNK12 is expressed mainly in the brain and lung, but also observed in the kidneys, heart and skeletal muscle. KCNK12 is closely related to KCNK13 (58% identity at the amino acid level), but could not yet been functionally expressed in vitro and may require other proteins to become active.
Background References:
- Rajan S, Wischmeyer E, Karschin C, et al. THIK-1 and THIK-2, a novel subfamily of tandem pore domain K+ channels. J. Biol. Chem. 2001; 276:7302-11
- Jezzini SH and Moroz LL. Identification and distribution of a twopore domain potassium channel in the CNS of Aplysia californica. Brain Res. Mol. Brain Res. 2004; 127:27-38
- Theilig F, Goranova I, Hirsch JR, et al. Cellular localization of THIK-1 (K(2P)13.1) and THIK-2 (K(2P)12.1) K channels in the mammalian kidney. Cell Physiol. Biochem. 2008; 21:63-74.
- Girard C, Duprat F, Terrenoire C, et al. Genomic and functional characteristics of novel human pancreatic 2P domain K+ channels. Biochem. Biophys. Res. Commun. 2001; 282:249-56.
Buffer:
KCNK12 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
KCNK12 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human KCNK12.
The immunogen is located within amino acids 320 - 370 of KCNK12.
The immunogen is located within amino acids 320 - 370 of KCNK12.
NCBI Gene ID #:
56660
NCBI Official Name:
potassium channel, subfamily K, member 12
NCBI Official Symbol:
KCNK12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_071338
Protein GI Number:
11545761
Purification:
KCNK12 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
KCNK12 antibody is predicted to not cross-react with other KCNK protein family members.
Swissprot #:
Q9HB15
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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