CHD7 Antibody
| Code | Size | Price |
|---|
| PSI-6441-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-6441-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
CHD7 Antibody: CRG, HH5, IS3, KAL5, KIAA1416, Chromodomain-helicase-DNA-binding protein 7, ATP-dependent helicase CHD7, CHD-7
Application Note:
CHD7 antibody can be used for detection of CHD7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
CHD7 Antibody: CHD proteins belong to a superfamily of proteins of ATP-dependent chromatin remodeling enzymes that have a unique combination of functional domains, including two N-terminal chromodomains, a SNF2-like ATPase/helicase domain and a DNA-binding domain. These proteins are thought to play a role in early embryonic development by affecting chromatin structure and gene expression. Mutations in one member of this family, CHD7, result in CHARGE syndrome. It colocalizes with embryonic stem (ES) cell master regulators OCT4/POU5F1, SOX2, and NANOG and is thought to modulate ES-specific gene transcription. Together with SOX2, CHD7 has been suggested to also regulate several human disease genes.
Background References:
- Woodage T, Basrai MA, Baxevanis AD, et al. Characterization of the CHD family of proteins. Proc. Natl. Acad. Sci. USA 1997; 94:11472-7.
- Cavalli G and Paro R. Chromo-domain proteins: linking chromatin structure to epigenetic regulation. Curr. Opin. Cell Biol. 1998; 10:354-60.
- Vissers LE, van Ravenswaaj CM, Admiraal R, et al. Mutations in a new member of the chromodomain gene family cause CHARGE syndrome. Nat. Genet. 2004; 36:955-7.
- Schnetz MP, Handoko L, Akhtar-Zaidi B, et al. CHD7 targets active gene enhancer elements to modulate ES cell-specific gene expression. PLoS Genet. 2010; 6:31001023.
Buffer:
CHD7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
CHD7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CHD7.
The immunogen is located within amino acids 670 - 720 of CHD7.
The immunogen is located within amino acids 670 - 720 of CHD7.
NCBI Gene ID #:
55636
NCBI Official Name:
chromodomain helicase DNA binding protein 7
NCBI Official Symbol:
CHD7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q9P2D1
Protein GI Number:
54112403
Purification:
CHD7 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
Multiple isoforms of CHD7 are known to exist.
Swissprot #:
Q9P2D1
User NOte:
Optimal dilutions for each application to be determined by the researcher.
Related Products
| Product Name | Product Code | Supplier |
|---|