Paralemmin3 Antibody

Contact us
ProSci
Product Code: PSI-6449
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6449-0.02mg0.02mg£150.00
Quantity:
PSI-6449-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

1 / 3
Western blot analysis of Paralemmin3 in MCF7 cell lysate with Paralemmin3 antibody at 1 μg/mL.
2 / 3
Immunocytochemistry of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 20 μg/mL.
❮❯

Western blot analysis of Paralemmin3 in MCF7 cell lysate with Paralemmin3 antibody at 1 μg/mL.
Immunocytochemistry of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 5 μg/mL.
Immunofluorescence of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
Paralemmin3 Antibody: Paralemmin-3
Application Note:
Paralemmin3 antibody can be used for detection of Paralemmin3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Paralemmin3 Antibody: Paralemmin3 is a recently identified protein that associates with the single immunoglobulin IL-1 receptor-related molecule (SIGIRR), a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Silencing of Paralemmin3 expression by RNAi inhibited the release of inflammatory cytokines in A549 cells after stimulation with lipopolysaccharide (LPS), suggesting Paralemmin3 may function as an adaptor in the LPS-TLR4 signaling pathway.
Background References:
  • Chen X, Wu X, Zhao Y, et al. A novel binding protein of single immunoglobulin IL-1 receptor-related molecule: Paralemmin-3. Biochem. Biophys. Res. Commun. 2011; 404:1029-33
  • Wald D, Qin J, Zhao Z, et al. SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Nat. Immunol. 2003; 4:920-7.
Buffer:
Paralemmin3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (79%)
Immunogen:
Paralemmin3 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human Paralemmin3.

The immunogen is located within amino acids 50 - 100 of Paralemmin3.
NCBI Gene ID #:
342979
NCBI Official Name:
paralemmin 3
NCBI Official Symbol:
PALM3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_001138500
Protein GI Number:
222537747
Purification:
Paralemmin3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
SPECIFICITY:
At least two isoforms of Paralemmin3 are known to exist; this antibody will only detect the longer isoform
Swissprot #:
A6NDB9
User NOte:
Optimal dilutions for each application to be determined by the researcher.

Related Products

Product NameProduct CodeSupplier